[Objective] Isolated bacterial strains which can resistance to root-knot nematodes and promote the growth of plant under the sub-low temperature condition. [Methods] Joint method was used to screen the bacterial strain, the joint method include nematodes resistance test, indoor low temperature resistance test, antagonism test and pot experiment. Phenotypic characteristic, physiological and biochemical characteristics and 16S rRNA gene sequencing method were used to identify the strains which passed screening method. [Results] 297 different bacteria and actinomyces strains were isolated from the soil of tomato and cucumber rhizosphere which are often seriously in root-knot nematode disease; After analysis the nematodes resistance rate, 9 strains which have more than 70% corrected death rate to the nematodes were isolated; A strain called S205 was isolated, it have excellent function in prevention and cure of root-knot nematode, soil-borne pathogens and promoting plant growth under sub-low temperature; Strain S205 was classified as Streptomyces antibioticus. [Conclusion] This research had isolated a biocontrol strain S205 which have the property of resistance root-knot nematode and promoting plant growth under sub-low temperature. The research is significant in prevention of root-knot nematode disease under sub-low temperature.