WSWXTB微生物学通报Microbiology China0253-2654微生物学通报编辑部中国北京wswxtb-48-11-405410.13344/j.microbiol.china.210195基因克隆及功能研究Microbial Genetics草菇过氧化氢酶基因(VvCAT1)异源表达及耐温度胁迫功能分析Expression of catalase gene (VvCAT1) from Volvariella volvacea in Escherichia coli and its temperature tolerance杨焕玲YANGHuanling常婷婷CHANGTingting赵妍ZHAOYanjiandan289@126.com*游华芳YOUHuafang查磊ZHALei李正鹏LIZhengpeng仝宗军TONGZongjun陈明杰CHENMingjiemjchen@saas.sh.cn*上海市农业科学院食用菌研究所 上海 201403Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
E-mail:赵妍:jiandan289@126.com
Catalase (CAT) is involved in the growth and development of fungi and protects them from oxidative damage in the event of stress.
Objective
The VvCAT1 gene from Volvariellavolvacea was expressed in Escherichia coli Stbl3 and its temperature tolerance was characterized.
Methods
VvCAT1 was cloned to construct a recombinant plasmid pBAR GPE1/VvCAT1 which was then transformed into Stbl3 to yield a recombinant strain expressing catalase. Catalase activity and growth of the recombinant strain (pBAR GPE1/VvCAT1/Stbl3) and the control strain (pBAR GPE1/Stbl3) were measured to verify the temperature tolerance.
Results
The recombinant strain exhibited stronger heat and cold tolerance than the control with increased catalase activity and better growth.
Conclusion
The transgenosis and expression of VvCAT1 significantly improved the heat and cold tolerance of E. coli Stbl3.
草菇过氧化氢酶异源表达耐温性Volvariellavolvaceacatalaseheterologous expressiontemperature tolerance上海市科技兴农项目2020-02-08-00-12-F01479上海市科技兴农项目(2020-02-08-00-12-F01479)Shanghai Agricultural Applied Technology Development Program2020-02-08-00-12-F01479Shanghai Agricultural Applied Technology Development Program (2020-02-08-00-12-F01479)
ReferencesLü BB, Wu X, Mo Q, Sun Y, Jiang W, Huang YN, Wu GG, Song LL, Wang JB, Li P, et alInvestigation of the key potential pathway involved in the early response to low temperature challenge in Volvariella volvacea202140510741086
Lü BB, Wu X, Mo Q, Sun Y, Jiang W, Huang YN, Wu GG, Song LL, Wang JB, Li P, et al. Investigation of the key potential pathway involved in the early response to low temperature challenge in Volvariella volvacea[J]. Mycosystema, 2021, 40(5): 1074-1086 (in Chinese)
Liu Z, Zhang K, Lin JF, Guo LQBreeding cold tolerance strain by chemical mutagenesis in Volvariella volvacea20111301182410.1016/j.scienta.2011.06.020
Liu Z, Zhang K, Lin JF, Guo LQ. Breeding cold tolerance strain by chemical mutagenesis in Volvariella volvacea[J]. Scientia Horticulturae, 2011, 130(1): 18-24
Hu LF, Yang YG, Jiang LW, Liu SQThe catalase gene family in cucumber: genome-wide identification and organization201639340841510.1590/1678-4685-GMB-2015-0192
Hu LF, Yang YG, Jiang LW, Liu SQ. The catalase gene family in cucumber: genome-wide identification and organization[J]. Genetics and Molecular Biology, 2016, 39(3): 408-415
Zámocký M, Gasselhuber B, Furtmüller PG, Obinger CMolecular evolution of hydrogen peroxide degrading enzymes2012525213114410.1016/j.abb.2012.01.017
Zámocký M, Gasselhuber B, Furtmüller PG, Obinger C. Molecular evolution of hydrogen peroxide degrading enzymes[J]. Archives of Biochemistry and Biophysics, 2012, 525(2): 131-144
Chen JF, Wang GN, Cheng SMProgress about catalase function in plant stress reactions200828118819310.3321/j.issn:1000-4025.2008.01.033
Chen JF, Wang GN, Cheng SM. Progress about catalase function in plant stress reactions[J]. Acta Botanica Boreali-Occidentalia Sinica, 2008, 28(1): 188-193 (in Chinese)
Saxena I, Srikanth S, Chen ZCross talk between H2O2 and interacting signal molecules under plant stress response20167570
Saxena I, Srikanth S, Chen Z. Cross talk between H2O2 and interacting signal molecules under plant stress response[J]. Frontiers in Plant Science, 2016, 7: 570
Liu YF, Wang WW, Zu YX, Mei Y, Zheng JQ, Wu YC, Guo J, Chen ZBResearch progress on the effects of catalase on plant stress tolerance201936158
Liu YF, Wang WW, Zu YX, Mei Y, Zheng JQ, Wu YC, Guo J, Chen ZB. Research progress on the effects of catalase on plant stress tolerance[J]. Barley and Cereal Sciences, 2019, 36(1): 5-8 (in Chinese)
Jiang W. Analysis of gene expression level and activity of key antioxidant enzymes from Volvariella volvacea in response to cold stress[D]. Nanjing: Master's Thesis of Nanjing Agricultural University, 2014 (in Chinese)
Yang HL, You HF, Zhao Y, Pattana K, Chang TT, Yu CX, Chen MJHeterologous expression of manganese superoxide dismutase from Volvariella volvacea in E. coli and characterization of its stress tolerance20202745158
Yang HL, You HF, Zhao Y, Pattana K, Chang TT, Yu CX, Chen MJ. Heterologous expression of manganese superoxide dismutase from Volvariella volvacea in E. coli and characterization of its stress tolerance[J]. Acta Edulis Fungi, 2020, 27(4): 51-58 (in Chinese)
Xin MM. The gene expression and function study of hydrophobin and heat shock proteins from Lentinula edodes[D]. Nanjing: Master's Thesis of Nanjing Agricultural University, 2016 (in Chinese)
Cooke BMethods of enzymatic analysis (third english edition) by HU Bergmeyer198816183183
Cooke B. Methods of enzymatic analysis (third english edition) by HU Bergmeyer[J]. Biochemical Education, 1988, 16: 183-183
Deng TT, Li J, Ma G, Wang XL, Zhao XL, Zhang FW, Qiao DR, Cao YIdentification of the function of superoxide dismutase gene from Dunaliella salina in SOD deleted strain K12200744117618010.3969/j.issn.0490-6756.2007.01.038
Deng TT, Li J, Ma G, Wang XL, Zhao XL, Zhang FW, Qiao DR, Cao Y. Identification of the function of superoxide dismutase gene from Dunaliella salina in SOD deleted strain K12[J]. Journal of Sichuan University: Natural Science Edition, 2007, 44(1): 176-180 (in Chinese)
Yang HL. Evaluation and application of high temperature resistant mycelium of Lentinula edodes[D]. Nanjing: Master's Thesis of Nanjing Agricultural University, 2018 (in Chinese)
杨焕玲. 香菇耐高温菌株的评价与应用[D]. 南京: 南京农业大学硕士论文, 2018
Li EYIntroducing wheat catalase to cultivate low temperature tolerance rice2001348
Li EY. Introducing wheat catalase to cultivate low temperature tolerance rice[J]. Biotechnology Information, 2001(3): 48 (in Chinese)
李恩义导入小麦过氧化氢酶培育耐低温水稻2001348
李恩义. 导入小麦过氧化氢酶培育耐低温水稻[J]. 生物技术通报, 2001(3): 48
Cai YZ, Zhu JB, Hao XY, Wang AY, Xu DXDrought resistance of KatG transgenic cotton20132212566110.7606/j.issn.1004-1389.2013.12.009
Cai YZ, Zhu JB, Hao XY, Wang AY, Xu DX. Drought resistance of KatG transgenic cotton[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2013, 22(12): 56-61 (in Chinese)
Ge F. Genetic transformation and functional characterization of pitaya CAT gene in tobacco[D]. Guiyang: Master's Thesis of Guizhou University, 2016 (in Chinese)
Polidoros AN, Mylona PV, Scandalios JGTransgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant-pathogen interactions and resistance to oxidative stress200110655556910.1023/A:1013027920444
Polidoros AN, Mylona PV, Scandalios JG. Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant-pathogen interactions and resistance to oxidative stress[J]. Transgenic Research, 2001, 10(6): 555-569
Yong B, Wang XY, Xu P, Zheng HY, Fei XT, Hong ZX, Ma QQ, Miao YZ, Yuan XH, Jiang YS, et alIsolation and abiotic stress resistance analyses of a catalase gene from Ipomoea batatas (L.) lam20176847532
Yong B, Wang XY, Xu P, Zheng HY, Fei XT, Hong ZX, Ma QQ, Miao YZ, Yuan XH, Jiang YS, et al. Isolation and abiotic stress resistance analyses of a catalase gene from Ipomoea batatas (L. ) lam[J]. BioMed Research International, 2017, 2017: 6847532
Su YC, Guo JL, Ling H, Chen SS, Wang SS, Xu LP, Allan AC, Que YXIsolation of a novel peroxisomal catalase gene from sugarcane, which is responsive to biotic and abiotic stresses201491e8442610.1371/journal.pone.0084426
Su YC, Guo JL, Ling H, Chen SS, Wang SS, Xu LP, Allan AC, Que YX. Isolation of a novel peroxisomal catalase gene from sugarcane, which is responsive to biotic and abiotic stresses[J]. PLoS One, 2014, 9(1): e84426
Guan DP. Study on physiological change of mycelium of various edible fungi under environment stress[D]. Fuzhou: Master's Thesis of Fujian Agriculture and Forestry University, 2004 (in Chinese)
Zhang GL. Effect of low temperature treatment on physiological biochemical indexes and nutrient accumulation in Pleurotus ferulae mycelia[D]. Urumqi: Master's Thesis of Xinjiang Agricultural University, 2013 (in Chinese)
Fu YP, Liang Y, Dai YT, Yang CT, Duan MZ, Zhang Z, Hu SN, Zhang ZW, Li YDe novo sequencing and transcriptome analysis of Pleurotus eryngii subsp. tuoliensis (bailinggu) mycelia in response to cold stimulation201621556010.3390/molecules21050560
Fu YP, Liang Y, Dai YT, Yang CT, Duan MZ, Zhang Z, Hu SN, Zhang ZW, Li Y. De novo sequencing and transcriptome analysis of Pleurotus eryngii subsp. tuoliensis (bailinggu) mycelia in response to cold stimulation[J]. Molecules, 2016, 21(5): 560
Wang LN. Characterization and function analysis of catalase genes in Pleurotus ostreatus[D]. Beijing: Doctoral Dissertation of Chinese Academy of Agricultural Sciences, 2019 (in Chinese)
Wang LN, Wu XL, Gao W, Zhao MR, Zhang JX, Huang CYDifferential expression patterns of Pleurotus ostreatus catalase genes during developmental stages and under heat stress201781133510.3390/genes8110335
Wang LN, Wu XL, Gao W, Zhao MR, Zhang JX, Huang CY. Differential expression patterns of Pleurotus ostreatus catalase genes during developmental stages and under heat stress[J]. Genes, 2017, 8(11): 335
Zhao Y, Lin F, Jiang W, Yan SY, Chen MJSequence analysis of cat1 and its relative expression in Volvariella volvacea under low temperature stress2015367196199, 275
Zhao Y, Lin F, Jiang W, Yan SY, Chen MJ. Sequence analysis of cat1 and its relative expression in Volvariella volvacea under low temperature stress[J]. Science and Technology of Food Industry, 2015, 36(7): 196-199, 275 (in Chinese)