CRISPR, a gene editing technology, has become a powerful molecular technology which has been increasingly applied in scientific research and clinical trials. In order to solve the problems of high complexity in principle, difficulties in operation, high time consumption and economic cost, we have constructed a virtual simulation teaching software of CRISPR gene editing technology that can be utilized in the experimental teaching. The system consists of two modules, which are principle demonstration and operation training. In the principle part, the structure of each molecule is presented by 3D technology, and the molecular mechanism of CRISPR-Cas9 is displayed in the form of animation. The practical operation part simulates the operation process of the whole experiment, and students can learn and operate repeatedly on the computer in the form of interaction. This interactive virtual experiment can stimulate students’ interest in learning, as well as significantly improving the quality of experimental teaching.