The type VI secretion system (T6SS) is a contractile secretion machinery with a needle-like structure, and capable of delivering effectors that can play a role in host colonization and niche establishment. In recent years, the presence of a functional T6SS in Campylobacter jejuni has been reported. However, the effect of T6SS to the virulence factors of C. jejuni were still unclear. This review provided the recent studies on T6SS of C. jejuni, including the structure components, prevalence, and function, which is to reveal the molecular mechanism for virulence factors of C. jejuni.