Microbiology is a discipline on the sciences of microbial life and the technologies of microbial application. In order to deeply grasp microbiological knowledge, proficiently master experimental skills and clearly understand social hot topics in limited class hours for the students with different academic backgrounds and different development visions, the curriculum reform of Microbiology has been carried out since 2013 in Zhejiang University. The results showed that the teaching quality was significantly improved by means of changing educational philosophy, integrating curriculum ideology, strengthening practice skills and emphasizing evaluation orientation.