Myxobacteriales belongs to Deltaproteobacteria, which is an important group of pharmaceutical-producing microorganisms. However, the isolation and purification of myxobacteria are difficult, which severely limits the exploration and utilization of myxobacteria resources. Myxobacteria are microbial predators, which produce a variety of extracellular hydrolases, such as amylase, protease, chitinase, cellulase, phosphatase, protease etc., to lyse other microorganisms or decompose cellulose as nutrients source. At present, the isolation and purification technology of myxobacteria are mainly based on predator-prey interaction or cellulose induction. Hereby, extracellular hydrolase of myxobacteria is the material basis for studying its isolation and cultivation methods. However, the hydrolases produced by myxobacteria have been less concerned. In this paper, the types, properties and functions of the hydrolases produced by myxobacteria are summarized, which would provide reference sense for the related researches on the isolation and cultivation techniques. Moreover, the exploitation and utilization of myxobacteria resources were also discussed.