Streptomyces are gram-positive bacteria with complex life cycle, and considered as the most extensive source of natural antibiotics due to the abundant and diverse secondary metabolites. Current studies on Streptomyces mainly focus on the secondary metabolism and morphological differentiation processes. The transport systems of Streptomyces have raised a lot of attention in recent years due to the important roles in these life processes. The transport systems have a great application prospect in the industrial production for their participation in morphological differentiation process through the signal transduction system and delivery of the secondary metabolites. In addition, as the “first door” of bacteria, transport systems can carry nutrients to regulate the primary metabolic processes, further affecting the processes of morphological differentiation and secondary metabolism, resulting in quick adaptation to the complex environment. Therefore, the studies on the transport systems of Streptomyces can provide important guidance for the interpretation of the regulation pathway of morphological differentiation.