In recent years, the nanotechnology has provided a variety of nanoscale materials for enzyme immobilization. The immobilized enzyme of nanomaterials not only has high enzyme load, but also has excellent enzyme stability. Based on the immobilized enzyme of nanomaterials, this paper summarized the types of nanomaterials, and the effects of nanomaterials on the performance of immobilized enzymes were analyzed. Immobilized methods and application of nanobiocatalysis in the fields of biological transformation, biosensors and biofuel cells were also introduced.