As the fundamental courses of environmental, biological, food and medical majors and so on, microbiological courses are extremely important. Within the “Internet +” era, modern educational technology accelerates the development of new form teaching remarkably. While bringing vitality into teaching activities, it effectively promotes educational equity, reduces teaching costs, and provides a realistic basis and reliable guarantee for the practice of lifelong learning. Connotative development puts forward new requirements to the construction of curriculum resource. In this paper, current situation and existing problems around construction of microbiological courses in colleges and universities were analyzed. Combining our practical experience on the construction and application of auxiliary book-network-mobile terminal integrated expansion resource, how high-quality teaching resource, especially expansion resource match well with high-quality course, could be built up was preliminary explored. This paper serves as a modest spur to induce someone to come forward with his valuable contributions.