In the current era of globalization, it is very important to promote English teaching for international cooperation and communication. However, how to avoid the English teaching becoming formalistic is a serious question. It is worth exploring to efficiently integrate English communication with knowledge impartment, so that the English teaching can really achieve the goals of building up a system of microbial knowledge, enhancing students’ interests, inspiring scientific thinking and knowledge application. Over the past few years, we adopted a gradual way to transit from a bilingual teaching of Microbiology to the whole English teaching of Microbiology genetics. The teaching contents are structured in ways that are from superficial to deep, from general to specific. Meanwhile, we devoted the teaching to the combination of basic knowledge and experimental technology. A various forms of teaching such as class test, after-school homework and class analysis and discussion have been involved to improve the teaching quality. After several years of continuous exploration and practice, English teaching has mobilized students’ active participation and learning, enabling students to exercise their ability to learn, explore, solve problems and communicate in English. The stimulation of students’ learning interest and enthusiasm demonstrated the effectiveness of the English teaching.