Facing the new challenges and new requirements of Microbiology Experimental teaching in TCM (Traditional Chinese Medicine) colleges and universities, the new contents and new methods of experimental teaching in the course of Microbiology Experiment teaching are put forward. Based on the internet, the three major network teaching platforms are established, and a variety of teaching resources and methods are applied to integrate the experimental teaching contents. In addition, the modular experimental teaching mode, experimental projects in research experiment, so as to improve the teaching quality of Microbiology Experiment course, cultivate the students? scientific thinking ability, innovation ability and team spirit and improve the quality of students? comprehensive objective.