Interdisciplinary comprehensive curriculum reform and comprehensive practice ability have become one of the hotspots of education reform. To enhance food microorganism examination course to learn professional skills, training of competitive talents needed for the development of industry, a cross-disciplinary collaborative teaching and the integration of WIKI website system was used for teaching reform and new pattern exploration in food microbiological examination course. The SPSS statistical analysis results on the teaching effect of 2014 school year and 2015 school year showed that it is significantly different that the average score of the students in 2015 was higher than the students in 2014. And 82.8% of the students considered that the new teaching model is useful to improve their professional ability. Practice proved that the new teaching pattern received remarkable achievements on strengthening students’ mastery of the knowledge base, extension professional knowledge, promoting students’ critical thinking as well as the ability of information literacy analysis. The reformed training is expected in line with the level of social development of professional knowledge and comprehensive ability of the quality of dual professional talents, which also provides a good new education mode for professional course teaching reform.