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微生物学通报

我国2002−2016年间鸡传染性支气管炎病毒基因组序列重组分析
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国家基础研究计划项目(No. 2013FY113300-4);国家重点研发计划项目(No. 2016YFD0500803);江苏省优势学科项目;江苏省动物重要疫病与人兽共患病防控协同创新中心项目


Recombination analysis of genomic sequences of infectious bronchitis viruses from 2002−2016 in China
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    摘要:

    【目的】传染性支气管炎病毒(Infectious bronchitis virus,IBV)主要引起鸡群呼吸道与肾脏疾病,是影响养禽业重要的病毒性病原之一。了解我国IBV的流行及基因重组情况。【方法】收集GenBank中我国2002?2016年间分离的92株IBV S1基因及55株IBV基因组序列,并对这些序列进行比对分析。【结果】IBV S1基因序列分析结果表明,2002?2016年间我国流行的92株IBV可以分为13个基因型,包括QX、4/91、Mass、tl/CH/LDT3/03、CK/CH/LSC/99I、TW-I、TW-II、TC07-2、Ck/CH/LDL/97I、N1/62-associated、Arkansas、New-I及一个新鉴定的基因分支New-II。值得注意的是,属于美国相关基因分支的ck/CH/LSD/110712已在我国出现。RDP4方法进行重组分析显示,新出现的基因分支New-II病毒S1基因来源于tl/CH/LDT3/03型与QX型毒株重组,我国2002?2016年间流行的55株IBV中有52株IBV基因组存在重组事件,其中25个IBV分离株基因组中发现有疫苗型(Mass,tl/CH/LDT3/03及4/91型等)病毒基因组片段的重组,这一结果在SimPlot分析中进一步得到确认。【结论】根据生物信息学分析结果,证明流行于我国的IBV基因型众多,疫苗毒株基因频繁参与了IBV基因重组,导致IBV新的基因型或变异株出现,提示在防控IB时要注意合理使用IBV疫苗。

    Abstract:

    [Objective] Infectious bronchitis virus (IBV) that causes respiratory and nephritic diseases in chicken is one of the important viral pathogens in poultry industry. To understand the epidemiology and recombination of IBV in China. [Methods] S1 gene sequences of 92 IBV strains and the genomic sequences of 55 strains isolated during 2002?2016 in China were collected from GenBank and analyzed by bioinformatics. [Results] Thirteen genotypes of IBV, including QX, 4/91, Mass, tl/CH/LDT3/03, CK/CH/LSC/99I, TW-I, TW-II, TC07-2, Ck/CH/LDL/97I, N1/62-associated, Arkansas, New-I and a newly emerging genotype New-II, were found co-circulation in China during 2002?2016 based on phylogenetic analysis of S1 gene. It was notable that ck/CH/LSD/110712 belonging USA branch was found in China in this study. Recombination analysis revealed the S1 gene of New-II genotype IBVs was originated from recombination between tl/CH/LDT3/03- and QX-type IBVs. With analysis of whole genomes of 55 IBV isolates, 52 recombinants were identified through RDP4. The partial sequence of vaccine strains were found in 25 of 52 IBV isolates. The recombination events were further confirmed by SimPlot bioinformatics analysis. [Conclusion] Bioinformatics analysis results demonstrated that multiple genotypes of IBV were co-circulated in China during 2002?2016, and novel IBV genotypic clusters and variants could be emerged through recombination which frequently occurring between field and vaccine strains. It indicates the adverse effects of IBV vaccine on controlling IB in China should be paid more attention.

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周海生,张美红,田雪,武奇,邵红霞,钱琨,叶建强,秦爱建. 我国2002−2016年间鸡传染性支气管炎病毒基因组序列重组分析[J]. 微生物学通报, 2017, 44(12): 2942-2950

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  • 在线发布日期: 2017-12-05
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