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微生物学通报

不同光照强度对小球藻生长的影响
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国家973计划前期研究专项(No. 2014CB460601);国家科技合作与交流专项项目(No. 2014DFE70070);国家自然科学基金青年基金项目(No. 51509086);水体污染控制与治理科技重大专项(No. 2014ZX07104-005-01,2014ZX07104-005-02)


Effects of different light intensity on the growth of Chorella vulgaris
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    目的】优化小球藻的培养与提高资源化利用效率,得到不同光照强度对小球藻生长的影响规律。【方法】通过室内控制实验,设置0、1 000、3 000、5 500、7 000、9 000 lx 共6 组光照强度,在温度为20 ℃ 和曝气强度为20%的条件下,采用BG-11 培养基培养小球藻(Chorella vulgaris)至稳定生长,研究不同光照强度对小球藻生长的影响,建立光照强度与最大光密度ODmax、最大比增长率μmax 之间的抛物回归曲线。【结果】不同光照强度下,小球藻生长差异较大,培养初期叶绿素a 浓度迅速增加直至峰值,后期迅速降低,其中5 500 lx 强度下小球藻生长最好。藻类生长对溶解性总磷的消耗比溶解性总氮更大。【结论】光照强度(x)与ODmax 的拟合方程为:ODmax=-1E–07x2+0.001 6x+0.105 5 (R2=0.987 2,0≤x≤9 000 lx),光照强度(x)与μmax 的拟合方程为:μmax=-2E–08x2+0.000 3x-0.004 2 (R2=0.998 6,0≤x≤9 000 lx)。

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    [Objective] This study provides the theoretical basis for optimal cultivation and nutrient utilization of Chorella vulgaris. The study aimed to discuss the effect of varying light intensity (0, 1 000, 3 000, 5 500, 7 000, 9 000 lx) on the growth of Chorella vulgaris. [Methods] The experiment was conducted under aeration rate of 20% and at 20 ℃, and the Chorella vulgaris was cultivated on the BG-11 culture medium under stable condition. Parabolic regression curves between light intensity and the maximum optical density (ODmax) and the maximum growth rate (μmax) were created. [Results] The growth rates varied different in different light intensity, and the chlorophyll a concentrations increased in the initial time until reached the maximum value and then decreased dramatically. The intensity of 5 500 lx was most suitable for the Chorella vulgaris growth. The demand of phosphorus of algal growth was greater than the nitrogen. [Conclusion] The mathematical relationships between light intensity and ODmax, μmax simulated by cubic curve equation were shown as follows: ODmax=-1E–07x2+0.001 6x+0.105 5 (R2=0.9872, 0≤x≤9 000 lx), μmax=-2E–08x2+0.000 3x-0.004 2 (R2=0.998 6, 0≤x≤9 000 lx).

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徐慧,纪道斌,崔玉洁,龙良红,刘德富. 不同光照强度对小球藻生长的影响[J]. 微生物学通报, 2016, 43(5): 1027-1034

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  • 在线发布日期: 2016-05-09
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