噬菌体展示技术是一种将外源肽或蛋白质与特定噬菌体衣壳蛋白相融合，展示于噬菌体表面来构建蛋白质或多肽文库，并从中筛选目的蛋白、多肽或抗体的基因工程高新技术。噬菌粒/辅助噬菌体系统是最常用的噬菌体展示系统，此系统中辅助噬菌体对噬菌粒的复制和组装发挥着至关重要的作用。本文结合当今该领域的最新研究动态，概述了噬菌粒和辅助噬菌体双基因组系统，着重介绍了不同辅助噬菌体的特点及其突变机制，并对其应用前景进行了展望，以期为该技术的进一步完善提供一定的借鉴作用。

Phage display is a widely used gene engineering high technology. Through the display of exogenous peptides or proteins fused specific coat protein on the surface of phages, it is possible to construct proteins or peptides libraries and screen interesting proteins, peptides and antibodies successfully. Most commonly used phage display technology is phagemid/helper phage system, in which helper phages are essential for the replication and assembly of phagemid particles. In this review, in combination with the newest research dynamic status, we summarize phagemid/helper phage double-genome system. We mainly emphasized the features and mutation mechanisms of different helper phages. We also made some prospects for the future directions, in the meanwhile, we also expect that our experience can provide some help for further maturity of the technology.

湖南省自然科学基金资助项目(No. 05JJ30065)