| 具有乙酰胆碱酯酶抑制活性的蛇足石杉内生真菌分子鉴定 |
| Molecular identification of endophytic fungi with inhibitory activity against acetylcholinesterase from Huperzia serrata |
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| 中文关键词:蛇足石杉，暗球腔菌科，系统发育分析 |
| 英文关键词:Huperzia serrata, Phaeosphaeriaceae, phylogenitic analysis |
| 基金项目:国家自然科学基金（No. 21066014，No. 81260617，No. 31300051）；国家“十二五”科技支撑项目（No. 2011BAC13B04）；“赣鄱英才555工程”领军人才计划；江西省重大科技专项（No. 2010AZD00307）；江西省教育厅科学技术研究项目（No. GJJ14595）；江西省研究生创新基金（No. YC2013-S106）；江西省亚热带植物资源保护与利用重点实验室开发基金（No. YRD201306，No. YRD201401） |
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| 中文摘要: |
| 在马铃薯葡萄糖液体培养基（PDB）、沙氏液体培养基（SDB）和查氏液体培养基（CDB）中发酵培养蛇足石杉内生真菌菌株SF88和LF52，乙酰胆碱酯酶（AChE）抑制活性检测结果表明，菌株SF88和LF52在PDB、SDB和CDB发酵后菌体总生物碱对AChE活性的抑制率分别为45.6%和56.7%、56.3%和68.5%、40.8%和48.3%。对这两株不产孢内生真菌的DNA ITS、18S rDNA、28S rDNA、TUB和RPB进行了测序，系统发育分析表明，SF88和LF52为Phaeosphaeriaceae科中一未知种，为开发生物碱类乙酰胆碱酯酶抑制剂（AChEI）药物提供了新的潜在菌种资源。 |
| 英文摘要: |
| Two endophytic fungus strains, SF88 and LF52, from Huperzia serrata were cultured in potato dextrose broth (PDB), sabouraud dextrose broth (SDB) and cazpek dextrose broth (CDB) liquid medium and the acetylcholinesterase- inhibitory activity of total alkaloid was analyzed. It was indicated that the inhibition rate of total alkaloid extracted from the strains SF88 and LF52 mycelium in PDB, SDB and CDB medium against acetylcholinesterase was 45.6% and 56.7%, 56.3% and 68.5%, 40.8% and 48.3%, respectively. rDNA ITS, 18S rDNA, 28S rDNA, TUB and RPB sequence analyses showed that the endophytic fungus strains belong to an unknown species in the family Phaeosphaeriaceae and SF88 and LF52 could be provided as the new potential resources for producing acetylcholinesterase-inhibitor. |
| 赖政,汪娣,汪涯,颜日明,张志斌,高波良,朱笃. 具有乙酰胆碱酯酶抑制活性的蛇足石杉内生真菌分子鉴定[J]. 菌物学报, 2014, 33(4): 858-866 |
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