| 不同营养条件下斑玉蕈菌丝生长及产酶特性 |
| Characteristics of mycelial growth and enzyme production of Hypsizygus marmoreus under different nutritional conditions |
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| 中文关键词:斑玉蕈，菌丝生长速度，菌丝形态，酶活性 |
| 英文关键词:Hypsizygus marmoreus, mycelial growth rate, mycelial characters, enzymes activities |
| 基金项目:福建省发改委农业五新工程项目（闽发改委投资[2012]931号）；福建省高校产学合作科技重大项目（No. 2013N5101）；福建农林大学校杰出青年人才项目（No. xjq201209） |
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| 中文摘要: |
| 分析测定了不同营养条件下斑玉蕈菌丝形态特征、生长速度及产酶规律。低碳氮盐培养基上菌丝生长速度最快，但其菌丝非常稀疏，边缘不整齐，在整个生长阶段酶活力（包括漆酶、锰过氧化物酶、木素过氧化物酶、木聚糖酶、纤维素酶）较低，营养不足对该菌菌丝生长速度影响不明显，但对菌丝形态和酶活有很大的影响；低氮条件下最先产生木质素过氧化物酶，说明限氮条件可以刺激木质素过氧化物酶的产生；高无机盐条件下最先产生漆酶和锰过氧化物酶，但菌丝生长速度较慢，酶活性比较低，浓度过高会影响菌丝生长。结果表明，不同的营养条件对斑玉蕈的菌丝生长及多种酶活性有很大影响，这为斑玉蕈改变营养条件调节菌丝生长速度、菌丝形态以及基质降解提供了理论依据，同时对斑玉蕈栽培过程中基质的高效利用、缩短生产周期、降低生产成本具有重要的指导意义。 |
| 英文摘要: |
| The characteristic of mycelial growth and enzyme production of Hypsizygus marmoreus under different nutritional conditions were determined and analyzed. The growth rate was the highest under the conditions of low nitrogen and carbon and low inorganic salt medium, but the mycelia grew sparsely and the colony margin was irregular, producing enzymes including laccase, manganese peroxidase, lignin peroxidase, xylanase and celluase with low activities. It was suggested the medium types did not affect mycelial growth rate obviously, but nutritional deficiency affected on mycelial morphology and enzyme activities significantly. Lignin peroxidase was produced at first in low nitrogen medium, and its generation was stimulated in the limitation of nitrogen. Laccase and manganese peroxidase were produced at the earliest in high-inorganic salts medium, but the mycelial growth rate and the enzyme activities were low. High concentration of inorganic salt affected the mycelial growth. The results indicated that mycelial growth characters and the enzyme production of Hypsizygus marmoreus were affected greatly by different nutritional conditions. The result also showed the nutritional condition adjustment could be an important practical measure to promote efficiency in substrate utilization and shorten the production cycle, and reduce the production cost during the process of cultivation of Hypsizygus marmoreus. |
| 郭艳艳,阮玲云,冯宏昌,胡开辉,孙淑静. 不同营养条件下斑玉蕈菌丝生长及产酶特性[J]. 菌物学报, 2014, 33(3): 697-705 |
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