| 尖顶羊肚菌胞外多糖提取物对皮肤成纤维细胞增殖和衰老的影响 |
| Effects of exopolysaccharide extract from Morchella conica on proliferation and aging of human skin fibroblasts |
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| 中文关键词:尖顶羊肚菌，胞外多糖，人皮肤成纤维细胞，衰老，β-半乳糖苷酶，羟脯氨酸 |
| 英文关键词:Morchella conica, exopolysaccharide, human skin fibroblasts, aging, β-galactosidase, hydroxyproline |
| 基金项目:广州市科技计划项目（No. 2012J4300129） |
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| 中文摘要: |
| 不同浓度尖顶羊肚菌胞外多糖提取物作用人皮肤成纤维细胞（human skin fibroblasts，HSF），检测对HSF细胞形态、细胞增殖、衰老相关β-半乳糖苷酶活性、羟脯氨酸含量的影响，探究尖顶羊肚菌胞外多糖提取物对HSF增殖和衰老的影响。结果显示，125μg/mL尖顶羊肚菌胞外多糖提取物使HSF细胞活力增加了25.2%，羟脯氨酸含量增加了12.1%，β-半乳糖苷酶活性降低了48.1%。说明适宜浓度尖顶羊肚菌胞外多糖提取物具有促进HSF细胞增殖、胶原蛋白合成，延缓细胞衰老的作用。 |
| 英文摘要: |
| The effects of exopolysaccharide extract from Morchella conica (EEMC) on cell senescence were examined in human skin fibroblasts (HSF). Cell morphology, proliferation, senescence-associated β-alactosidase (SA-β-Gal) activity, and hydroxyproline content of cells treated by exopolysaccharide were measured. HSF cell viability and hydroxyproline content increased by 25.2% and 12.1% respectively, and β-galactosidase activity decreased by 48.1%, under the treatment of EEMC at the concentration of 125μg/mL. It was indicated that EEMC promoted the proliferation of HSF and the production of collagen, and thus deferred the cell aging. |
| 马利,李霞,张松. 尖顶羊肚菌胞外多糖提取物对皮肤成纤维细胞增殖和衰老的影响[J]. 菌物学报, 2014, 33(2): 385-393 |
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