| SSR在黑木耳和毛木耳转录组中的分布和序列特征 |
| Distribution and sequence characteristic of SSR in the transcriptomes of Auricularia auricula-judae and Auricularia polytricha |
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| 中文关键词:黑木耳，毛木耳，转录组，简单重复序列，基序 |
| 英文关键词:Auricularia auricula-judae, A. polytricha, transcriptome, SSR, motif |
| 基金项目:中国博士后科学基金（No. 20110491164） |
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| 中文摘要: |
| 以黑木耳和毛木耳转录组测序获得的转录本序列为基础，采用软件SSR Locator对两个物种中SSR的数量、分布和结构特征等方面进行分析，发现毛木耳中SSR的丰度和密度较黑木耳小。在两者的SSR中，三碱基和六碱基重复的出现最多。对含量最多的三碱基重复序列的基序进行分析表明富含GC的SSR在这两个种的转录组中占优势地位。 |
| 英文摘要: |
| Illumina Solexa sequencing technology was used to generate transcript sequences of Auricularia auricula-judae and A. polytricha. The number, distribution and motif characteristic of SSR in this two species were analyzed using the software SSR Locator. The SSR abundance and density in A. polytricha were less than that in A. auricula-judae. However, tri- and hexa-nucleotide SSR were two kinds of significantly abundant repeats in both transcriptomes. The motif analysis of tri-nucleotide SSR which was the most popular repeats indicated the GC-rich repeats were the dominant SSR in both transcriptomes. |
| 周雁,范秀芝,陈连福,边银丙. SSR在黑木耳和毛木耳转录组中的分布和序列特征[J]. 菌物学报, 2014, 33(2): 280-288 |
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