| 人工栽培绣球菌的鉴定及其子实体β-葡聚糖含量的酶法测定 |
| Molecular identification of cultivated Sparassis species and determination of β-glucan content of the fruit body by using enzymatic method |
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| 中文关键词:广叶绣球菌，β-葡聚糖，冷冻干燥，烘干干燥 |
| 英文关键词:Sparassis latifolia, β-glucan, freeze-drying, hot air-drying |
| 基金项目:国家自然科学基金（No. 31100014）；中国科学院微生物研究所真菌学国家重点实验室资助 |
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| 中文摘要: |
| 在对中国福建省栽培的绣球菌进行种类鉴定的基础上，采用国际通用的酶法测定绣球菌子实体β-葡聚糖含量，研究了干燥方式对其含量的影响，并与一些常见食药用真菌的β-葡聚糖含量进行比较。结果显示该栽培绣球菌为广叶绣球菌Sparassis latifolia，子实体柄部和瓣片部分的β-葡聚糖含量均超过了40%，柄部的含量显著高于瓣片部分，冻干或烘干对其含量没有显著影响。广叶绣球菌β-葡聚糖含量极显著高于猴头、香菇、蛹虫草和草原黄蘑菇。 |
| 英文摘要: |
| The ribosomal DNA internal transcribed spacer (rDNA ITS) region was amplified and sequenced to identify the species of artificial cultured Sparassis. The β-glucan content of fruit body of Sparassis was determined and compared with other edible fungi using enzymatic method. The effects of two different drying methods including freeze-drying and hot air-drying on the β-glucan content from Sparassis were also assessed. The results showed that the species of Sparassis cultivated in Fujian Province was S. latifolia. The β-glucan content of both stipes and flabellae of S. latifolia was over 40%, and that of stipes was significantly higher than that of flabellae. There was no significant difference between β-glucan content of freeze-drying fruit body and that of hot air-drying one. It was indicated that the β-glucan content from S. latifolia was significantly higher than that of Hericium erinaceus, Cordyceps militaris, Lentinula edodes and Armillaria luteovirens. |
| 廉添添,杨涛,孙军德,黄贤华,董彩虹. 人工栽培绣球菌的鉴定及其子实体β-葡聚糖含量的酶法测定[J]. 菌物学报, 2014, 33(2): 254-261 |
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