人工栽培绣球菌的鉴定及其子实体β-葡聚糖含量的酶法测定
Molecular identification of cultivated Sparassis species and determination of β-glucan content of the fruit body by using enzymatic method
  
中文关键词:广叶绣球菌,β-葡聚糖,冷冻干燥,烘干干燥
英文关键词:Sparassis latifolia, β-glucan, freeze-drying, hot air-drying
基金项目:国家自然科学基金(No. 31100014);中国科学院微生物研究所真菌学国家重点实验室资助
作者单位
廉添添 中国科学院微生物研究所真菌学国家重点实验室 北京 100101 沈阳农业大学土地与环境学院 辽宁 沈阳 110866 
杨涛 中国科学院微生物研究所真菌学国家重点实验室 北京 100101 
孙军德 沈阳农业大学土地与环境学院 辽宁 沈阳 110866 
黄贤华 福建容益菌业科技研发有限公司 福建 福州 350100 
董彩虹 中国科学院微生物研究所真菌学国家重点实验室 北京 100101 
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中文摘要:
      在对中国福建省栽培的绣球菌进行种类鉴定的基础上,采用国际通用的酶法测定绣球菌子实体β-葡聚糖含量,研究了干燥方式对其含量的影响,并与一些常见食药用真菌的β-葡聚糖含量进行比较。结果显示该栽培绣球菌为广叶绣球菌Sparassis latifolia,子实体柄部和瓣片部分的β-葡聚糖含量均超过了40%,柄部的含量显著高于瓣片部分,冻干或烘干对其含量没有显著影响。广叶绣球菌β-葡聚糖含量极显著高于猴头、香菇、蛹虫草和草原黄蘑菇。
英文摘要:
      The ribosomal DNA internal transcribed spacer (rDNA ITS) region was amplified and sequenced to identify the species of artificial cultured Sparassis. The β-glucan content of fruit body of Sparassis was determined and compared with other edible fungi using enzymatic method. The effects of two different drying methods including freeze-drying and hot air-drying on the β-glucan content from Sparassis were also assessed. The results showed that the species of Sparassis cultivated in Fujian Province was S. latifolia. The β-glucan content of both stipes and flabellae of S. latifolia was over 40%, and that of stipes was significantly higher than that of flabellae. There was no significant difference between β-glucan content of freeze-drying fruit body and that of hot air-drying one. It was indicated that the β-glucan content from S. latifolia was significantly higher than that of Hericium erinaceus, Cordyceps militaris, Lentinula edodes and Armillaria luteovirens.
廉添添,杨涛,孙军德,黄贤华,董彩虹. 人工栽培绣球菌的鉴定及其子实体β-葡聚糖含量的酶法测定[J]. 菌物学报, 2014, 33(2): 254-261
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