| 球孢白僵菌继代培养中的菌落局变现象 |
| STRAIN VARIATION OF BEAUVERIA BASSIANA IN SUBCULTURING |
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| 中文关键词:球孢白僵菌 菌落局变 继代培养 |
| 英文关键词:Beauverin bassiana Saltation Subculture |
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| 中文摘要: |
| 通过球孢白僵菌两个野生型菌株及其单孢株在不同培养基、温度、湿度和光照条件下继代培养,结果产生3种不同类型的菌落局变.局变产生的分离子多表现为菌落瘩薄或气生菌丝陡长、产孢量下降、生长速率增加等现象.培养基、温度、湿度和光照等因素对菌落局变有明显诱导作用.湿度为15℃,相对湿度较低及全光照条件下于SDAY培养基上培养,局变发生频率最低.在实验室继代培养中,野生型菌株在4-14代内即可发生局变而被局变分离子所取代.引人注意的是,单孢株局变产生的分离子同时伴随着棕黄色或红棕色素的分泌,仅以菌落形态和颜色等培养特征作为球孢白僵菌种的划分依据是不可靠的.文中提出了菌落局变发生的遗传变异可能机制. |
| 英文摘要: |
| Two wild-type multi-spore isolates of Beauveria bassiana and some single spore isolates from them were subcultured on 7 artificial media under different conditions of temperature, humidity and light. The results showed that substantial saltation of 3 different types occured mainly due to different growth rates and morphologies of original colony and sectorial variation area. The variation of morphology, color, growth rate and sporulation of these segregants derived from the sectors under different conditions was tested and compared. The initial wild- type isolates degenerated within 4-14 generations. Medium, temperature, humidity, and light induced the variation obviously. Cultures on SDAY at 15℃, comparatively low humidity, and under full light resulted in low frequency variation. Noticebly, segregants derived from the single-spore isolates produced brownish yellow or reddish brown pigment secretion, suggesting the unreliablity of specy division by culture characteristics such as colony color and morphology. In addition, growth rate increase was followed by sporulation decline. They were negatively correlated. Some possible approach to the strain variation were proposed involving genetic mechanisms inner and outer nuclei. |
| 唐晓庆,樊美珍,李增智. 球孢白僵菌继代培养中的菌落局变现象[J]. 菌物学报, 1995, 14(Z1): 137-151 |
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