| 真菌miRNA的发现 |
| miRNA in the fungus Cryptococcus neoformans |
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| 中文关键词:miRNA，新型隐球酵母，RNA干扰 |
| 英文关键词:miRNA, Cryptococcus neoformans, RNAi |
| 基金项目:国家自然科学基金（No. 3170138，No. 31100061） |
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| 摘要点击次数: 1976 |
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| 中文摘要: |
| microRNAs（miRNAs）在植物和动物中大量存在，但是否在真菌中存在一直是个未解之谜。本研究组在担子菌新型隐球酵母Cryptococcus neoformans中发现了miRNA。两个miRNA，miR1和miR2，长度分别是22nt和18nt，前体是70nt，和动物miRNA相近。通过报告基因，证实miR1/2具有沉默功能。真菌miRNA的发现为研究其进化、功能等提供有用知识。 |
| 英文摘要: |
| Small endogenous microRNAs (miRNAs), known as posttranscriptional repressors in plants and animals, have mostly been thought lost in the Kingdom of Fungi. Here, we describe the identification of miRNAs from Cryptococcus neoformans. With bioinformatics and Northern blotting approaches, we found the miRNAs and their hairpin precursors in this fungus. The size of miR1 and miR2 are 22nt and 18nt, respectively. The precursors are about 70nt that is close to mammalian pre-miRNAs. We demonstrated that miR1 and miR2 caused transgene silencing via the canonical RNAi pathway. Numerous possible targets of miR1/2 are located number of identical sequences of the miR1/2 in transposable elements (TEs) and pseudogenes, indicating that fungal miRNAs might be involved in the regulation of the activity of transposons and the expression of pseudogenes. |
| 胡阳,姜楠,黄怡文,朱旭东. 真菌miRNA的发现[J]. 菌物学报, 2013, 32(Z1): 62-69 |
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