| 肌动蛋白基因和β-微管蛋白基因用于真黏菌系统发育的可行性研究 |
| The feasibility of using actin gene and beta-tubulin gene in phylogenetic analysis of Myxomycetes |
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| 中文关键词:肌动蛋白基因，β-微管蛋白基因，无丝菌目，团毛菌目，绒泡菌目，发网菌目 |
| 英文关键词:actin gene, β-tubulin gene, Liceales, Trichiales, Physarales, Stemonitales |
| 基金项目:国家自然科学基金（No. 30970019）；江苏省高校自然科学研究重大项目（No. 12KJA180004） |
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| 中文摘要: |
| 真黏菌是一类独特的菌物。目前对其进行的系统发育研究主要是基于形态特征，分子水平的系统发育研究上小亚基核糖体RNA基因和蛋白质合成延长因子基因研究相对较多。为了扩充能有效地进行真黏菌系统发育研究的基因资源，探讨了肌动蛋白基因和β-微管蛋白基因用于真黏菌系统发育的可行性。共获得14个基因序列，肌动蛋白基因和β-微管蛋白基因各7个。在GenBank中除多头绒泡菌Physarum polycephalum外并无其他真黏菌的肌动蛋白基因和β-微管蛋白基因序列，研究获得的14个基因序列为真黏菌基因的新序列。系统发育分析表明，肌动蛋白基因能够有效地将无丝菌目、团毛菌目、绒泡菌目和发网菌目区分为4个分支，其中发网菌目为一个独立的进化支，支持了根据子实体发育所认识的真黏菌纲内部具有两条进化路线的观点，因此显示出肌动蛋白基因对于真黏菌系统发育研究的重要价值。 |
| 英文摘要: |
| The Myxomycetes is a unique class of fungi. Until now, the systemic studies of myxomycetes has been mainly based on the morphological characteristics, while the molecular phylogenetic analysis has mainly been inferred from small subunit rRNA and elongation factor 1-α gene sequences. In this study, actin gene and β-tubulin gene sequences were analysed for determining if they can be effectively applied to the study on myxomycete phylogeny. Seven actin gene sequences and seven β-tubulin gene sequences were respectively obtained from field-collected fruiting bodies of eight myxomycete species. The fourteen sequences were firstly reported. Prior to this paper, available data of actin gene and β-tubulin gene sequences were quite limited because there was not any other actin or β-tubulin gene sequences of Myxomycetes in GenBank except those of Physarum polycephalum. The results showed that actin gene can effectively dividing the Liceales, Trichiales, Physarales and Stemonitales into four branches, and the order Stemonitales displayed alone in an evolutionary branch. The results supported the viewpoint proposed according to the formation of fruiting body, i.e., the class Myxomycetes had historically consisted of two phylogenetic routes. This also suggested that actin gene have an important significance in phylogenetic analysis of Myxomycetes. |
| 方玲,刘歧莎,李倩,闫淑珍,李玉,陈双林. 肌动蛋白基因和β-微管蛋白基因用于真黏菌系统发育的可行性研究[J]. 菌物学报, 2013, 32(6): 1004-1011 |
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