| 三种木层孔菌子实体不同溶剂提取物抗氧化活性的研究 |
| Antioxidant activities of different fractional extracts from fruiting bodies of three Phellinus species |
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| 中文关键词:桑木层孔菌，鲍姆木层孔菌，瓦宁木层孔菌，不同提取物，抗氧化活性 |
| 英文关键词:Phellinus mori, Phellinus baumii, Phellinus vaninii, fractional extracts, antioxidant activities |
| 基金项目:教育部新世纪优秀人才支持计划（No. NCET-11-0585）；国家自然科学基金（No. 30870013, No. 30910103907） |
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| 中文摘要: |
| 以抗坏血酸为阳性对照，分别对桑木层孔菌Phellinus mori、鲍姆木层孔菌Phellinus baumii和瓦宁木层孔菌Phellinus vaninii野生子实体甲醇提取物（ME）、氯仿提取物（CE）、乙酸乙酯提取物（EAE）、正丁醇提取物（BE）和热水提取物（WE）的体外清除DPPH（1,1-二苯基-2-三硝基苯肼）自由基、羟自由基和总抗氧化能力进行了检测。结果表明，3种木层孔菌的不同溶剂提取物都具有体外抗氧化活性。3种菌EAE清除DPPH自由基能力较强，其中瓦宁木层孔菌EAE清除DPPH自由基能力最强，IC50为10.65μg/mL。3种木层孔菌热水浸提物清除羟自由基的能力较强，鲍姆木层孔菌WE的清除率最高，1mg/mL时达到73.52%。另外，3种木层孔菌不同提取物总抗氧化能力和清除DPPH自由基能力基本相同，瓦宁木层孔菌BE的总抗氧化能力最强，1mg/mL时达到了167.7U/mL。3种木层孔菌相比，瓦宁木层孔菌不同提取物的体外抗氧化能力最强，其次是鲍姆木层孔菌，桑木层孔菌的体外抗氧化能力最弱。 |
| 英文摘要: |
| The in vitro antioxidant activities of the methanol extract (ME), chloroform extract (CE), ethyl acetate extract (EAE), butanol extract (BE) and hot water extract (WE) from fruiting bodies of Phellinus mori, P. baumii and P. vaninii were investigated. The results indicated that all fractional extracts from the fruiting bodies of these species had in vitro antioxidant activities. EAE from Phellinus vaninii had the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity as compared with the other fractional extracts, and IC50 values reached 10.65μg/mL. The hot water extracts (WE) of them all displayed higher hydroxyl radical (·OH) scavenging activity, and the WE from Phellinus baumii showed the highest effect reaching 73.52% at 1mg/mL. The total antioxidant capacities of fractional extracts from the fruiting bodies of these species were similar to the DPPH radical scavenging activity. The total antioxidant capacity of BE from Phellinus vaninii reached 167.7U/mL, ranking the highest among these species. As for the total capacity of antioxidant activities of the extracts of all the solvents tested, Phellinus vaninii ranked first, and next came P. baumii and P. mori. |
| 曹春蕾,韩美玲,崔宝凯,余海尤. 三种木层孔菌子实体不同溶剂提取物抗氧化活性的研究[J]. 菌物学报, 2013, 32(5): 883-890 |
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