| 耐镉马克思克鲁维酵母重金属镉吸附特性的研究 |
| The characteristics of cadmium adsorption by Cd-resisting Kluyveromyces marxianus YS-K1 |
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| 中文关键词:马克思克鲁维酵母YS-K1，镉，能量抑制剂，锌，吸附，积累 |
| 英文关键词:Kluyveromyces marxianus, cadmium, metabolic inhibitors, zinc, biosorption, bioaccumulation |
| 基金项目:国家高技术研究发展计划（863计划）（No. 2006AA06Z353）；中央高校基本科研业务费专项资金（No. SWJTU11ZT25） |
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| 中文摘要: |
| 菌株YS-K1是从镉污染土壤中筛选获得的一株耐镉马克思克鲁维酵母Kluyveromyces marxianus YS-K1，对其镉吸附特性研究结果表明，该菌对镉具有良好的耐受性和吸附性，即使在镉浓度高达140mg/L的培养基中也能生长，当镉浓度在30mg/L以下时，24h后能吸附溶液中90% 以上的镉，且最佳吸附条件为pH6.0，温度30℃。该菌所吸附的镉大部分集中在细胞壁上，占总吸附镉的76%，细胞膜上的占9%，细胞质中的占15%；能量抑制剂2,4-二硝基苯酚（DNP）和二环己基碳二亚胺（DCC），分别能降低菌株15%和22%的镉吸附量。在Kluyveromyces marxianus YS-K1细胞中，锌离子与镉离子有共用的结合位点，锌离子不仅会与镉离子竞争细胞表面的结合位点，减少其对镉的吸附量，而且也会与镉离子竞争进入细胞的通道位点，减少其对镉的积累量，同时能部分恢复镉离子对菌株生长的抑制作用。 |
| 英文摘要: |
| The characteristics of cadmium adsorption by Kluyveromyces marxianus YS-K1, a Cd-resisting yeast isolated from polluted soil, were studied. The results showed that Kluyveromyces marxianus YS-K1 had high resistance and effectual adsorption to cadmium. Even under cadmium concentration of 140mg/L it could grow up well. Under concentration below 30mg/L, the strain could also adsorb more than 90% of the cadmium in 24 hour incubation under the optimal pH value of 6.0 and the optimal temperature of 30℃. 76% of adsorbed cadmium was concentrated on cell wall, 9% on membrane and 15% in cytoplasm. The cadmium adsorption reduced 15% and 22% under the treatment of metabolic inhibitors DNP and DCC. Zinc not only competed with cadmium for cell surface binding sites, and reduced biosorption ability of Kluyveromyces marxianus YS-K1 to cadmium, but also competed in occupying the binding sites of entrance passageway of the cells, reducing bioaccumulation of cadmium, and recovering the inhibition activity of cadmium to the strain. |
| 王小波,李学如,茆灿泉,江南屏,郭泰林. 耐镉马克思克鲁维酵母重金属镉吸附特性的研究[J]. 菌物学报, 2013, 32(5): 868-875 |
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