网柄细胞状黏菌纯化培养基的改进 |
Improvement of purified media of dictyostelid cellular slime molds |
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中文关键词:网柄菌,培养,纯化 |
英文关键词:dictyostelids, culture, purification |
基金项目:国家自然科学基金(No. 31170012,No. 31093440,No. 30770005) |
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中文摘要: |
网柄细胞状黏菌传统的分离培养是在添加细菌的培养基上进行的。选取了9种网柄细胞状黏菌作为研究对象,在不饲喂任何细菌的条件下于3种培养基上进行培养,通过对生长状态的比较,发现燕麦片琼脂培养基对于纯化网柄细胞状黏菌具有较好的效果。 |
英文摘要: |
Feeding bacteria on the culture medium was a traditional methods used to isolate dictyostelid cellular slime molds. Under the conditions without feeding any bacteria, 9 dictyostelid species were selected and cultured in 3 kinds of media. It was showed that oatmeal agar medium was the optimal medium to purify dictyostelids. |
安颖,刘朴,祁亮亮,李玉. 网柄细胞状黏菌纯化培养基的改进[J]. 菌物学报, 2013, 32(4): 771-775 |
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