| 内生真菌Preussia sp.液体发酵生产抗肿瘤活性产物Spiropreussione A的研究 |
| The production of Spiropreussione A, an antitumor metabolite produced by endophytic fungus Preussia sp., with liquid fermentation |
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| 中文关键词:内生真菌，代谢产物，抗肿瘤活性，发酵条件优化 |
| 英文关键词:endophytic fungus, metabolite, cytotoxicity, optimization of fermentation conditions |
| 基金项目:国家自然科学基金项目“一种真菌抗艾滋病病毒（HIV）活性成分研究”（No. 31170016）；科技部“重大新药创制”专项“创新药物候选物研究”（No. 2012ZX09301-002-001） |
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| 中文摘要: |
| 螺光黑壳菌酮A（Spiropreussione A，SP-A）是编号为AS-5的光黑壳属内生真菌Preussia sp.的代谢产物。体外实验表明SP-A对人卵巢癌细胞A2780和人肝癌细胞BEL-7404的半数抑制浓度（IC50）分别为2.4和3.0μmol/L。以SP-A的含量为主要指标，结合AS-5的生物量，通过单因素实验和正交实验，优化确定了适合SP-A积累的AS-5液体发酵培养基和培养条件。研究结果表明：AS-5发酵生产SP-A的最优培养基为葡萄糖2%，麦麸3%，磷酸二氢钾0.3%，硫酸镁0.15%，pH 7.0；该菌株最佳摇瓶发酵条件为250mL摇瓶装125mL培养基，接种6片直径9mm的PDA菌片，培养温度25℃，发酵周期16d。在此条件下发酵，SP-A的含量可以达到(25.02±1.02)mg/瓶，比优化前的含量[(17.08±3.24)mg/瓶]提高了46.5%。 |
| 英文摘要: |
| Spiropreussione A (SP-A), a metabolite of endophytic fungus Preussia sp. AS-5, showed cytotoxicity in vitro experiment toward A2780 and BEL-7404 cells with IC50 values of 2.4 and 3.0μmol/L, respectively. The objective of this research was to optimize the medium and the liquid fermentation condition of isolate AS-5 for the production of SP-A by single factor and orthogonal tests. The results showed that the best medium for SP-A production contained glucose 2%, wheat bran 3%, KH2PO4 0.3%, and MgSO4 0.15%; the initial pH was 7.0. The optimum fermentation conditions were: inoculating 6 pieces of PDA fungal plates (Φ9mm) in 250mL Erlenmeyer flask containing 125mL medium, and incubating the fungus under the temperature of 25℃ for 16 days. Under such a condition, the content of SP-A reached (25.02±1.02)mg/flask and increased by 46.5% as compared to that before optimization [(17.08±3.24)mg/flask]. |
| 陈晓梅,施琦渊,王春兰,李兵,王爱荣,郭顺星. 内生真菌Preussia sp.液体发酵生产抗肿瘤活性产物Spiropreussione A的研究[J]. 菌物学报, 2013, 32(4): 729-740 |
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