| 分离自安徽土壤金龟子绿僵菌种群的遗传异质性研究 |
| Genetic heterogeneity of Metarhizium anisopliae populations from the soils collected from Anhui Province |
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| 中文关键词:虫生真菌，ISSR分子标记，基因流，遗传分化 |
| 英文关键词:entomopathogenic fungi, inter-simple sequence repeat (ISSR), gene flow, genetic differentiation |
| 基金项目:国家自然科学基金（No. 30972368）；安徽省高校优秀人才基金项目（No. 2012SQRL061）；农业部公益性行业专项（No. 201003079） |
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| 中文摘要: |
| 金龟子绿僵菌是昆虫种群自然控制和害虫生物防治中重要的虫生真菌，已被作为化学农药的替代品广泛应用于农林害虫的防治。其寄主范围和地理分布极广，因此种群结构也十分复杂。为明确安徽省土栖金龟子绿僵菌的种群遗传结构，采用ISSR分子标记技术对采集自安徽省不同地区土壤中的116株金龟子绿僵菌进行遗传异质性分析。分子数据显示，筛选出的8个引物共获得79个位点，其中多态位点比率为100%。不同地区种群的Nei’s基因多样性（H）为0.2796，Shannon信息指数（I）为0.4425，种群间存在一定程度的基因流（Nm=4.4282）和遗传分化（Gst=0.1015），种群内的基因多样度占总居群的89.85%，种群间占10.15%，表明安徽土栖金龟子绿僵菌的遗传变异主要来源于种群内，且种群内表现出较大的遗传变异。并采用UPGMA对所有供试材料进行聚类分析，得到7大类种群。结果表明，安徽土栖金龟子绿僵菌之间的亲缘关系与地理来源不存在相关性。 |
| 英文摘要: |
| Metarhizium anisopliae is an important entomopathogenic fungus for natural population regulation and biological pest control against different insects. The fungus has been developed into promosing alternatives to chemical insecticides in the programs of controlling agricultural and forestry insect pests. It has broad host range and wide geographic distribution, and hence complicated population genetic structures. In order to clarify the genetic differentiation of M. anisopliae population isolated from the soil samples in Anhui, the genetic heterogeneity of 116 isolates from different areas of Anhui Province was investigated using ISSR. In total, 79 fragments were amplified using eight primers screened previously, and the results showed 100% of polymorphism for the eight primers. For the populations from different areas, the indices of Nei’s gene diversity (H) was estimated to be 0.2796, Shannon’s diversity index (I) 0.4425, coefficient of gene differentiation (Gst) 0.1015, and gene flow (Nm) 4.4282. The genetic diversity proportion among populations (10.15%) was lower than that within the populations (89.85%), suggesting that the genetic variation of M. anisopliae mainly came from the interior of the populations. The UPGMA cluster analysis showed that the genetic similarities among seven populations were not related with geographical origins. |
| 陈名君,周玉宝,黄勃. 分离自安徽土壤金龟子绿僵菌种群的遗传异质性研究[J]. 菌物学报, 2013, 32(2): 179-191 |
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