一株产生L-乳酸的米根霉 |
A Rhizopus oryzae strain producing L-lactic acid |
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中文关键词:米根霉,溴甲酚绿平板,摇瓶发酵试验 |
英文关键词:Rhizopus oryzae, bromocresol green agar, shaking flask test |
基金项目:湖南省教育厅资助项目(No. 06C090);湖南师范大学微生物分子生物学湖南省重点实验室开放基金(No. MM0802) |
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中文摘要: |
为获得理想的L-乳酸产生菌,选择适合根霉属微生物生长的土样,利用溴甲酚绿平板结合摇瓶复筛的方法得到了一株有一定L-乳酸积累能力的米根霉Rhizopus oryzae CS323。摇瓶发酵试验显示,在未优化发酵条件的情况下发酵48h,米根霉CS323L-乳酸积累量达到50.1g/L,是一株有良好改造潜力的L-乳酸产生菌,适合作为进一步诱变育种的出发菌株。 |
英文摘要: |
In order to obtain an ideal strain producing L-lactic acid, Rhizopus oryzae strain CS323 was obtained by bromocresol green agar plate and shaking flask screening from soil samples. Shaking flask test showed that, without optimization of fermentation conditions, L-lactic acid accumulation of the strain CS323 was 50.1g/L after 48h fermentation. It was a potential L-lactic acid producer suitable for further mutation. |
周倩,赵龙,蒋雪薇,罗晓明,边佳为. 一株产生L-乳酸的米根霉[J]. 菌物学报, 2012, 31(6): 956-962 |
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