| 用灵芝发酵人参水煎液的工艺优化及其产物的抗疲劳活性研究 |
| Optimization of fermentation process of ginseng water decoction with Ganoderma lucidum strain and antifatigue activity of the fermentation product |
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| 中文关键词:红参，最佳培养基，抗疲劳，乳酸含量 |
| 英文关键词:red ginseng, optimum medium, antifatigue effect, contents of blood lactic acid |
| 基金项目:吉林省农业委员会（No. 212-00262） |
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| 中文摘要: |
| 以灵芝为发酵菌株，对其发酵所采用的基础培养基和人参水煎液培养基分别进行筛选，并对人参水煎液的灵芝升罐发酵工艺及其发酵产物的抗疲劳活性进行了研究，结果表明灵芝最适基础培养基为葡萄糖5%，玉米浆0.3%，豆饼粉1%，糖蜜0.6%，MgSO4 0.075%，KH2PO4 0.15%；最适人参水煎液培养基为红参水煎液，浓度为40g/L；灵芝在红参水煎液中升罐发酵的最佳条件为：发酵周期58–62h。在此优化条件下，菌丝体干重达到7.0233g/L，胞外粗多糖含量达到0.3167g/L。人参的灵芝发酵产物的高、低剂量能明显延长小鼠游泳耗竭时间，并且降低游泳休息后小鼠的血乳酸含量，其中低剂量与空白组有显著性差异（P<0.05），高剂量与空白组有极显著性差异（P<0.01）。说明红参水煎液的灵芝发酵产物具有显著的抗疲劳作用。 |
| 英文摘要: |
| The basal culture medium and ginseng water decoction medium were screened for Ganoderma lucidum fermentation, and antifatigue activity of G. lucidum-fermented ginseng water decoction was studied. The ingredients of optimum basal medium for G. lucidum was glucose 5%, corn steep liquor 0.3%, soybean cake powder 1%, molasses 0.6%, MgSO4 0.075% and KH2PO4 0.15%. The red ginseng water decoction medium with concentration of 40g/L was the optimum medium for the fermentation. The optimal fermentation period was 58–62h. Under these optimal conditions, the dry weight of the mycelium biomass reached 7.0233g/L, and the content of extracellular crude polysaccharide (ECP) was 0.3167g/L. As compared with the control group, in either case of low dose (P<0.05) and high dose (P<0.01) of red ginseng-G. lucidum fermentation product could significantly prolong the mice’s swimming depletion time and dramatically reduce the contents of blood lactic acid (BLA). Thus, the red ginseng-G. lucidum fermentation product showed obvious antifatigue effects. |
| 刘洋,李磊,昝立峰,包海鹰. 用灵芝发酵人参水煎液的工艺优化及其产物的抗疲劳活性研究[J]. 菌物学报, 2012, 31(6): 924-932 |
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