| 桦褐孔菌菌丝体及甾类化合物的发酵条件优化 |
| Optimization of submerged fermentation condition for production of mycelial biomass and steroids by Inonotus obliquus |
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| 中文关键词:桦褐孔菌，最佳发酵培养基，初始pH，接种量 |
| 英文关键词:Inonotus obliquus, optimum fermentation medium, initial pH, inoculation volume |
| 基金项目:国家“863”重点项目（No. 2011AA02A211）；江苏省自然科学基金（No. BK2010142）；江苏省产学研联合创新资金项目（No. BY2012052）；中央高校基础研究项目（Nos. JUSRP211A42, JUSRP211A43） |
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| 中文摘要: |
| 对桦褐孔菌深层发酵培养基进行了筛选，以菌丝体及甾类化合物产量为目标对发酵条件进行了优化，确定最佳发酵条件为：30g/L葡萄糖，2.5g/L黄豆粉，2.5g/L蛋白胨，3g/L KH2PO4，0.8g/L MgSO4，0.8g/L CaSO4，初始pH4.0，接种量15%，装液量100mL/500mL，转速150r/min，28℃恒温培养。此条件下培养11d，菌丝体干重达12.52g/L，甾体类化合物的产量达112.44mg/L。 |
| 英文摘要: |
| To produce mycelial biomass and steroids, the optimization of submerged fermentation condition for Inonotus obliquus was carried out. The optimum fermentation medium was as follows: 30g/L glucose, 2.5g/L Soybean powder, 2.5g/L peptone, 3g/L KH2PO4, 0.8g/L MgSO4, 0.8g/L CaSO4. The initial pH was 4.0, and the inoculation volume was 15%. The fungus was cultured at 28℃ in 500mL shake flasks containing 100mL of medium at 150r/min. Under this condition, mycelial biomass of 12.52g/L was obtained while production of steroids about 112.44mg/L. |
| 黄伟,陆震鸣,耿燕,许泓瑜,史劲松,许正宏. 桦褐孔菌菌丝体及甾类化合物的发酵条件优化[J]. 菌物学报, 2012, 31(6): 909-916 |
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