| 液体发酵法筛选樟芝优良诱变新菌株的研究 |
| Screening of Taiwanofungus camphoratus mutagenic strains by liquid fermentation |
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| 中文关键词:樟芝，物理诱变，多糖，三萜，蛋白质 |
| 英文关键词:Taiwanofungus camphoratus, physical mutation, polysaccharide, triterpenoid, protein |
| 基金项目:山东省科技攻关项目（No. 2010GNC10956） |
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| 中文摘要: |
| 对3个樟芝野生菌株和5个物理诱变菌株进行液体发酵，通过测定发酵液黏度变化情况，观测培养性状、菌球及粗提物产量和多糖、蛋白质及三萜含量，从中筛选出适宜液体发酵的多糖、三萜及蛋白质高产优良菌株。试验结果显示：供试的8个樟芝菌株生长曲线基本一致，其中，菌株327和Gg生长速度较快，培养的第9–10天开始出现菌丝自溶现象；经过筛选，327菌株为多糖和蛋白质高产菌株，多糖和蛋白质的产量分别比出发菌株提高238.20%和10.33%；Gg为三萜高产菌株，三萜产量比出发菌株提高57.86%。野生樟芝菌株经物理诱变效果明显。 |
| 英文摘要: |
| To obtain the high-yield strains producing polysaccharide, triterpenoid and protein suitable for liquid fermentation, three wild strains and five physical mutagenic strains of Taiwanofungus camphoratus were screened by liquid cultivation. The viscosity of the fermentation liquor was measured during the cultivation. The yields of mycelial pellets and extraction, the content of polysaccharide, triterpenoid and protein as well as cultural characteristics were determined after the termination of fermentation. The results showed that eight strains of T. camphoratus grew in a similar pattern, but strain 327 and Gg grew faster than other six tested strains. Mycelial autolysis were observed at the 9th–10thd. Strain 327 was the high-yielding strain producing polysaccharide and protein, with an increase of 238.20% and 10.33% as compared to original strain, respectively. Gg was selected as the high-yielding strain producing triterpenoid, and the yield of triterpenoid was increased by 57.86% as compared with the original strain. Significant efficacy was obtained by physical mutation strains in comparison with original strain. |
| 田雪梅,张国利,宋爱荣. 液体发酵法筛选樟芝优良诱变新菌株的研究[J]. 菌物学报, 2012, 31(6): 900-908 |
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