| Ganoderma lucidum U-281漆酶催化偶氮染料活性黑5脱色 |
| Decolorization of reactive black 5 catalyzed by laccase from Ganoderma lucidum U-281 |
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| 中文关键词:白腐真菌，合成染料，酶促氧化，脱色降解 |
| 英文关键词:white rot fungi, synthetic dyes, enzymatic oxidation, decoloration and degradation |
| 基金项目:安徽省高校省级自然科学研究项目（No. KJ2011B025）；安徽工程大学引进人才科研启动基金（No. 2007YQQ007） |
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| 中文摘要: |
| 漆酶在纺织染料脱色及印染废水处理领域有着广阔的应用前景。活性黑5是纺织印染中应用广泛的偶氮类活性染料，结构复杂，生物降解性低。以灵芝菌Ganoderma lucidum U-281所产漆酶对活性黑5进行氧化脱色，采用单因素逐一优化方法得到了U-281漆酶催化活性黑5脱色的工艺参数：染料初始浓度25mg/L、漆酶用量2.0U/mL、铜离子添加量40mmol/L、pH 6.0、40℃。在优化条件下，4h可使RB5脱色62.34%，24h可完成90%以上的脱色效果。 |
| 英文摘要: |
| Laccase has an extensive application on the decoloration of textile dyes and waste-dyeing-water treatment. Reactive black 5 (RB5), a representative azo dye, has complex structure and is low-biodegradable. In this paper, laccase produced by Ganoderma lucidum U-281 was used to decolorize RB5. The optimal parameters for RB5 decolorizing catalyzed by laccase from U-281 were seriatim obtained by single factor optimization method. The results were showed as follows: 25mg/L of initial dye concentration, 2.0U/mL of laccase dosage, 40mmol/L of Cu2+ addition and pH 6.0 at 40℃. Under the optimized conditions, 62.34% and more than 90% of RB5 was decolorized in 4 hours and 24 hours respectively. |
| 赵世光,张焱,薛正莲,王洲,汤明礼. Ganoderma lucidum U-281漆酶催化偶氮染料活性黑5脱色[J]. 菌物学报, 2012, 31(6): 867-877 |
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