| 正红菇菌丝体凝集素的分离纯化及生化特性 |
| Purification and biochemical properties of lectin from mycelium of Russula vinosa |
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| 中文关键词:正红菇凝集素，纯化，理化性质，抑菌活性 |
| 英文关键词:Russula vinosa lectin, purification, characterization, antimicrobial activity |
| 基金项目:福建省科技厅重点资助项目（No. 2008N0034）；福建师范大学生物学国家实验教学示范中心课题（No. 2009ls32） |
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| 中文摘要: |
| 正红菇菌丝体经磷酸缓冲液浸提、硫酸铵分级沉淀、DEAE-Sepharose FF离子交换层析和Sephadex G-100分子筛层析纯化得到红菇凝集素（Russula vinosa Lectin，RVL）。经SDS-PAGE检测为单一蛋白带，其亚基相对分子质量为55kDa，Sephadex G-100凝胶过滤测得相对分子质量为55.25kDa，提示RVL分子只有一个亚基。RVL中性糖含量为3.87%，经酸水解测定含15种氨基酸。温度在20–60℃、pH在5–9的范围内，凝集活性保持相对的稳定。RVL的凝血活性受Mn2+、Zn2+、Ca2+的影响。糖抑制实验表明，在供试的11种糖中，D-甘露糖强烈抑制RVL的凝血活性。抑菌实验显示，RVL对供试的细菌没有抑制作用，对稻瘟病菌、绿色木霉、红色链包霉、黑曲霉菌丝生长有显著的抑制作用。 |
| 英文摘要: |
| Russula vinosa Lectin (RVL) was obtained from Russula vinosa mycelium with the methods of extraction by phosphate buffer, precipitation in 40%–80% (NH4)2SO4 and the successive chromatography over DEAE-Sepharose Fast Flow and Sephadex G-100. It was indicated that RVL contained one subunit with the relative molecular mass of 55kDa determined by SDS-PAGE and 55.25kDa estimated by gel filtration. RVL consisted of 3.87% neutron-saccharide and 15 kinds of amino acid. The activity of RVL would remain stable at 20–60℃ and pH 5–9 and affected by demetalization and addition of divalent metals including Mn2+, Zn2+and Ca2+. D-mannose could significantly inhibit the activity of RVL in the carbohydrate inhibition assay which includes 11 kinds of sugar. The mycelial growth of Piricularia oryzae, Trichoderma viride, Neurospora crassa and Aspergullus niger could be obviously inhibited by RVL, while RVL had no inhibiting effects on tested bacteria. |
| 刘艳如,郑永标,黄维雅,林勇,吴小君,任海亮. 正红菇菌丝体凝集素的分离纯化及生化特性[J]. 菌物学报, 2012, 31(6): 857-866 |
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