| 尖镰孢古巴专化型1号生理小种致病相关基因敲除突变体△Focr1-328的生物学特性 |
| Biological characteristics of the pathogenic-related deletion mutant △Focr1-328 of Fusarium oxysporum f. sp. cubense race 1 |
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| 中文关键词:香蕉枯萎病，致病性，敲除突变体，生物学特性 |
| 英文关键词:banana Fusarium wilt, pathogenicity, knock out strain, biological characteristics |
| 基金项目:国家自然科学基金（No. 30860160）；国家公益性行业（农业）科研专项课题（No. 200903049-2）；海南大学“211工程”建设项目；海南大学环境与植物保护学院研究生创新平台项目 |
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| 中文摘要: |
| 由尖镰孢古巴专化型1号生理小种Fusarium oxysporum f. sp. cubense race 1（Focr1）引起的香蕉枯萎病是粉蕉类香蕉品种（AAB）不能规模化种植的最主要因素，其致病机理至今尚不十分清楚。本实验室前期通过T-DNA插入获得Focr1致病性丧失突变体Focr1-328，从Focr1全基因组序列中定位了因T-DNA插入失活的基因，并从野生型菌株Focr1-N2中敲除了该致病相关基因，获得了敲除突变体△Focr1-328。为了明确该致病相关基因的生物学功能，通过活体叶片、活体根 |
| 英文摘要: |
| Fusarium wilt of banana (Musa spp.), caused by Fusarium oxysporum f. sp. cubense race 1, is the most important factor limiting the plantation of plantain banana (AAB Group Fenjao) on the large scale. The pathogenic mechanism is still not very clear yet. A nonpathogenic mutant Focr1-328 with a T-DNA insertion was recently obtained. The inactivated gene resulting from a T-DNA insertion was located in the genomic sequences of Focr1. Subsequently, the gene was knocked out and a deletion mutant △Focr1-328 was obtained by homologous recombination. In order to investigate the function of the pathogenic related gene, the pathogenicity of the wild type Focr1-N2 and the deletion mutant △Focr1-328 was tested by inoculating living leaves and roots with spore suspension. The differences in the colony morphology, mycelial growth and conidial production on PDA, the growth rates and morphology on different carbon and nitrogen sources and the biomass, potential of hydrogen value, optical density value as well as cellophane penetrating capability were studied. The results of pathogenicity experiments showed that the deletion mutant △Focr1-328 was nonpathogenic, indicating that △Focr1-328 lost the pathogenic capability. The growth rate, the quantity and germination rate of conidia and the biomass of mycelia of the deletion mutant △Focr1-328 were significantly lower than those of wild type. The best carbon sources for the mutant and the wild type growth was sorbitol and maltose, respectively. The optimum Nitrogen source was sodium nitrate in both cases but the growth rates and colors of mutant and wild type were different on various C and N media. The wild strain penetrated cellophane readily, but the mutant could not. The results indicated that pathogenic related gene which was inactivated by T-DNA insertion is related to the utilization of C source, acid production and mycelial penetration capability. |
| 唐改娟,曾涛,曾会才,林妃,郭刚,彭明. 尖镰孢古巴专化型1号生理小种致病相关基因敲除突变体△Focr1-328的生物学特性[J]. 菌物学报, 2012, 31(4): 593-607 |
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