| 尖镰孢古巴专化型4号小种T-DNA插入突变体Focr4-1562及其基因敲除子的生物学表型研究 |
| Phenotype analysis of the T-DNA insertion mutant Focr4-1562 of Fusarium oxysporum f. sp. cubense race 4 and its gene-knockout mutant |
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| 中文关键词:香蕉枯萎病，T-DNA插入突变体，基因敲除，生物学特性 |
| 英文关键词:Banana wilt disease, T-DNA insertion mutant, gene knockout, biological characters |
| 基金项目:国家自然科学基金（No. 30860160）；国家公益性行业（农业）科研专项课题（No. 200903049-2）；海南大学“211工程”建设项目；海南大学环境与植物保护学院研究生创新平台 |
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| 中文摘要: |
| 尖镰孢古巴专化型4号小种Fusarium oxysporum f. sp. cubense race 4（Focr4）是引起毁灭性土传病害香蕉枯萎病的危害性最大的小种，至今其致病机理尚不清楚。对已获得的野生型菌株Focr4-193-6经基因T-DNA插入引起致病性严重减弱的突变体Focr4-1562及其插入失活基因的敲除子△Focr4-1562的生物学表型进行了研究。玻璃纸穿透试验、活体叶片接种及根部接种致病性测定的结果表明，插入突变体和敲除子不能穿透玻璃纸生长，叶片接种和根部接种未见有明显病斑和球茎维管 |
| 英文摘要: |
| Fusarium oxysporum f. sp. cubense race 4 (Focr4) is considered as one of the most destructive pathogens of the world distributed soil-borne banana. Its pathogenesis has not been clearly understood. The pathogenicity of mutant Focr4-1562 was significantly weakened after T-DNA insertion in its gene sequence. The inserted exogenous gene caused changes in functions of some genes in the △Focr4-1562. By means of cellophane penetration test as well as pathogenic test by inoculating live leaves and roots, changes in pathogenic phenotype of the mutant Focr4-1562 and △Focr4-1562 were compared with the wild type Focr4. The mutant Focr4-1562 and △Focr4-1562 could not grow through cellophane, and didn’t cause any disease spots and brown coloration in corm vascular bundle. The results showed that the optimum growth temperature of the wild type Focr4, Focr4-1562 and △Focr4-1562 were all at 28℃, and their optimum growth pH was 7.0 to 8.0. No evident difference was found in their spore morphology, but the sporulation of △Focr4-1562 was obviously delayed, and its sporulation capacity was significantly decreased. The Focr4-1562 and △Focr4-1562 were more resistant to cell wall degrading enzyme than the wild type Focr4-193-6. It is concluded that the knockout of the pathogenic gene is responsible for conidial production and cell wall formation of the wild type Focr4-193-6. |
| 吴飞宏,曾涛,陈汉清,曾会才,彭明. 尖镰孢古巴专化型4号小种T-DNA插入突变体Focr4-1562及其基因敲除子的生物学表型研究[J]. 菌物学报, 2012, 31(4): 584-592 |
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