| 稻曲病两个白化菌株的分离与生物学特性 |
| The isolation and biological features of two albinotic isolates of Ustilaginoidea virens |
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| 中文关键词:稻曲病菌，白化菌株，孢子形成，菌核 |
| 英文关键词:Ustilaginoidea virens, albinotic isolate, sporulation, sclerotium |
| 基金项目:公益性行业（农业）科研专项（No. 200903039-5）；水稻生物学国家重点实验室开放基金资助项目 |
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| 中文摘要: |
| 为了筛选带有自然标记的稻曲病菌菌株，2010年从浙江省象山县和陕西省勉县采集和分离到2个稻曲病白化菌株，ZJa0201和SXa0101。它们在PSA培养基上的生长速度约为其他稻曲病菌株的3倍，未见产生厚垣孢子；在PS培养基上只能产生少量分生孢子。rDNA-ITS和rDNA-IGS序列分析表明，两个白化菌株也与稻曲病菌已知所有菌株的ITS序列同源性高于99.6%；rDNA-IGS序列也属于最为常见的类型，含有2个77bp的重复单元序列。由此推断，这两个白化菌株属于稻曲病菌产孢退化的突变体。白化菌株在PSA上 |
| 英文摘要: |
| Two isolates of Ustilaginoidea virens with natural marker, ZJa0201 and SXa0101, were obtained from albinotic false smut balls of rice. The albinotic isolates grew rapidly and reached about 3 times the growth speed of wild isolates in potato sucrose agar (PSA) medium, and no chlamydospore was detected. Sporulation of the albinotic isolates decreased significantly in PSA medium. The analysis of rDNA-ITS sequences showed that they shared 99.6% similarity to the known wild isolates of U. virens, and their rDNA-IGS sequences belonged to the most common type, consisted of 2 repetitive units of 77bp in the central various region. It is suggested that these albinotic isolates were the mutants of sporulating degeneration of Ustilaginoidea virens. The albinotic isolates could produce immature sclerotia in 30 days in PSA and UV irradiation could promote the formation of selerotia. The immature sclerotia could not germinate. |
| 金菊,陈宏明,许伟,杨炳辉,胡东维. 稻曲病两个白化菌株的分离与生物学特性[J]. 菌物学报, 2012, 31(4): 567-573 |
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