| 中国北方棉花主产区立枯丝核菌的融合群鉴定 |
| Anastomosis groups of Rhizoctonia solani from cotton in northern China |
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| 中文关键词:立枯丝核菌，融合群，5.8S rDNA-ITS区序列，致病性 |
| 英文关键词:Rhizoctonia solani, anastomosis group, 5.8S rDNA-ITS sequence, pathogenicity |
| 基金项目:国家自然科学基金（No. 30870007）；山东省自然基金（No. Y2007D38）山东省现代农业产业技术体系 |
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| 中文摘要: |
| 从山东、河北、河南三省采集棉花立枯病样品和土壤200余份，经分离获得198个丝核菌Rhizoctonia solani分离物。菌丝融合测定及5.8S rDNA-ITS区序列分析结果表明，这些分离物分别属于多核丝核菌的AG4-HG-I和AG4-HG-III融合群以及双核丝核菌的AG-A、AG-F、AG-Fb融合群。其中AG4-HG-I是优势融合类群，占分离物总数的88.38%，其次是AG4-HG-III，占10.10%，AG-A、AG-F、AG-Fb各仅有1株。其中双核丝核菌AG-A、AG-F和AG-Fb融 |
| 英文摘要: |
| A total of 198 Rhizoctonia solani isolates were obtained from diseased cotton and soil samples in northern China (Shandong, Hebei and Henan provinces). Anastomosis group (AG) assay and 5.8S rDNA-internal transcribed spacer (ITS) sequence analysis showed the presence of multinucleate Rhizoctonia AG4-HG-I, AG4-HG-III and binucleate Rhizoctonia AG-A, AG-F and AG-Fb groups. The group AG4-HG-I was the dominant（86.86% of total isolates), followed by AG4-HG-III (10.10%), while the AG-A, AG-F, AG-Fb groups were represented by single isolate respectively. The Rhizoctonia AG-A, AG-F, AG-Fb groups were firstly reported from diseased cotton plants, and their pathogenicity to cotton needs further investigation. The 5.8S rDNA-ITS sequence analysis showed that the isolates could be distinctly separated based on their AG types. The isolates of the same AG (or sub-AG) showed high sequence identity. The nuclear phase observation showed that the numbers of nuclei were significantly different between the two subgroups of AG-4. |
| 井岩,李晓妮,于金凤. 中国北方棉花主产区立枯丝核菌的融合群鉴定[J]. 菌物学报, 2012, 31(4): 540-547 |
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