| 中国烟草根黑腐病菌根串珠霉菌群及rDNA-ITS序列分析 |
| Grouping and rDNA-ITS sequence analysis of Thielaviopsis basicola on tobacco in China |
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| 中文关键词:烟草根黑腐病，根串珠霉，厚垣孢子，分群，rDNA-ITS |
| 英文关键词:tobacco black root rot, Thielaviopsis basicola, chlamydospore, grouping, rDNA-ITS |
| 基金项目:重庆市自然科学基金（No. 2010BB1143）；中央高校基本科研业务费专项资金（No. XDJK2010C080）；烟草病虫害监测与综合治理重点开放实验室开放课题（No. BC201002） |
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| 中文摘要: |
| 从国内不同烟区分离获得烟草根黑腐病菌根串珠霉45个野生型和11个白色变异菌株，参照Punja & Sun的分群鉴定方法，对这56个菌株进行了分群鉴定。结果表明：中国根串珠霉分离株存在明显形态变异，共划分出6个群，其中群Ⅰ、Ⅱ、Ⅲ为野生型，群Ⅳ、Ⅴ为白色变异型；另有白色变异型HLL-1￠不同于其他各群，将其建为新群—群Ⅵ。统计分析发现，群Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ及Ⅵ的分离频率分别占42.9%、33.9%、3.6%、16.0%、1.8%和1.8%。群Ⅰ主要分布重庆和云南，群Ⅱ在5个调查省市均有分布，群Ⅲ、群Ⅴ仅分布 |
| 英文摘要: |
| Tobacco black root rot caused by Thielaviopsis basicola was frequently reported in China in recent years. In this study, a total of 45 isolates of wild types and 11 isolates of white aberrant phenotypes of the pathogen were collected from some provinces of China. According to Punja & Sun’s grouping standards, their grouping identification was conducted. The results showed that six general morphological groups could be distinguished among the isolates, in which groupsⅠ,Ⅱ and Ⅲ were wild types, groups Ⅳ andⅤ aberrant phenotypes. The characteristics of colony and chlamydospores of another aberrant phenotype HLL-1￠ was completely different from those of the five groups mentioned above, therefore it was assigned to the new group, group Ⅵ. The isolating ratios of groups Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ and Ⅵ were 42.9%, 33.9%, 3.6%, 16.0%, 1.8% and 1.8% respectively. Group Ⅰwas mainly distributed in Chongqing and Yunnan, group Ⅲ and Ⅴ in Guizhou, group Ⅳ mainly in Chongqing, Henan and Yunnan, group Ⅵ only in Henan, and group Ⅱ was distributed in all investigating provinces. The rDNA-ITS sequence analysis suggested that the white aberrant phenotypes were identical to their wild types in sequences, but completely different from the isolates growing on other host plants. |
| 窦彦霞,彭雄,余佳敏,高超,肖崇刚,周常勇. 中国烟草根黑腐病菌根串珠霉菌群及rDNA-ITS序列分析[J]. 菌物学报, 2012, 31(4): 531-539 |
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