| 中国落叶松-杨栅锈菌遗传多样性研究 |
| Genetic diversity of poplar rust fungus Melampsora larici-populina in China |
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| 中文关键词:落叶松-杨栅锈菌，遗传结构，SSR分子标记 |
| 英文关键词:Melampsora larici-populina, genetic structure, simple sequence repeat |
| 基金项目:国家自然科学基金（Nos. 30872027, 30471394） |
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| 中文摘要: |
| 采用SSR分子标记技术对采自全国19个地区、不同年份的落叶松-杨栅锈菌Melampsora larici-populina（简称MLP）36个单孢子堆菌系的遗传多样性和种群遗传结构进行了研究。用5对SSR多态性引物共检测到62个观察等位基因（Na），有效等位基因数（Ne）为5.42–9.82，平均有效等位基因数为7.05。供试MLP样本在5个SSR位点上的多态性信息量（PIC）变化范围为0.64–0.89，平均值0.79。平均观察杂合度（Ho）、平均期望杂合度（He）分别为0.36和0.86，不同位点的S |
| 英文摘要: |
| Melampsora larici-populina (abbr. MLP) is a heteroecious fungal pathogen causing foliar rust on Populus and Larix species. The genetic diversity of 36 isolates of MLP, collected from 19 locations of eight provinces in China in different years, was investigated by simple sequence repeat (SSR) analysis. A total of 62 alleles for five SSR loci were detected from 91 samples within 36 isolates of MLP, and the effective number of alleles (Ne) ranged from 5.42 to 9.82, with an average of 7.05. The polymorphism information content (PIC) for five loci ranged from 0.64 to 0.89, with an average of 0.79. The average values of observed heterozygosity (Ho) and expected heterozygosity (He) were 0.36 and 0.86, respectively, and Shannon’s information index (I) varied from 1.88 to 2.52, with an average of 2.12. These results showed that all the test MLP isolates were very rich in genetic diversity. The coefficient of gene differentiation among isolate populations (Fst) varied from 0.22 to 0.39, with an average of 0.344, and the average value of gene flow (Nm) was 0.48, which revealed that the genetic diversity appeared mainly within isolate populations (65.60%), specially between isolate populations of MLP from natural forest areas, but also showed distinct differentiation of genetics among isolate populations (34.40%). At geographical population level, the average I and Nei’s gene diversity (Nei’s) were 1.02 and 0.56, respectively, and average values of polymorphic loci (Np) and percentage of polymorphic loci (PPL) were 4.54 and 90.77%, respectively, implying that the genetic diversity among geographical populations was relatively lower than that within isolate populations of MLP. Based on Nei’s genetic distance, ranging from 0.24 to 2.99, 13 geographical populations of MLP isolates from different regions in China could be initially grouped into four clusters: cluster I included isolates By, Th, HD and Sb, cluster II included isolates Cj, HZ, Gl and Qh, cluster III included isolates Nm, Wh and Yl, and cluster Ⅳcomprised Ts and Tt. |
| 曹支敏,杜林,王秦虎,余仲东. 中国落叶松-杨栅锈菌遗传多样性研究[J]. 菌物学报, 2012, 31(4): 510-522 |
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