| 蛹虫草水溶性多糖含量测定方法的比较与优化 |
| Comparison and optimization of the methods for analyzing the water soluble polysaccharides in Cordyceps militaris |
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| 中文关键词:蛹虫草，多糖，苯酚硫酸法 |
| 英文关键词:Cordyceps militaris, polysaccharide, phenol-sulfuric acid procedure |
| 基金项目:上海市科技兴农重点攻关项目”香菇、灰树花、北冬虫夏草种质创新及其生产加工关键技术研究”[沪农科攻字（2009）第2-2号] |
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| 中文摘要: |
| 已报道文献中蛹虫草多糖含量差异非常大，通过比较分析发现差异主要在于干扰物质的去除。结合高效阴离子色谱对干扰物质的测定，对常见的几种蛹虫草多糖提取测定方法进行了分析比较，在此基础上建立了去除干扰物的蛹虫草多糖测定方法。该方法简便、快速、精确，经系统的方法学考察证明，是一种分析蛹虫草多糖较好的方法。 |
| 英文摘要: |
| There are significant differences of the polysaccharide content from Cordyceps militaris assayed with different methods in previous literatures, which could be mainly caused by removing the interfering substances or not. After monitoring the interfering substances by high performance anion exchange chromatography, two common analysis methods for determination of the polysaccharides of C. militaris were compared. We found that the extraction with 80% ethanol in twice could effectively remove the interfering substance. Our study showed that it was a sensitive, accurate and reproducible method and could be successfully applied to analyze the water soluble polysaccharides produced by C. militaris. |
| 薛俊杰,唐庆九,刘艳芳,周帅,赵明文,张劲松. 蛹虫草水溶性多糖含量测定方法的比较与优化[J]. 菌物学报, 2012, 31(3): 443-449 |
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