| 古尼拟青霉麦角甾醇高产液体培养条件及提取工艺的优化 |
| Optimization of liquid culture conditions and extraction process of ergosterols from Paecilomyces gunnii |
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| 中文关键词:古尼拟青霉，昆虫病原真菌，麦角甾醇，最适液体培养基，最适培养条件 |
| 英文关键词:Paecilomyces gunnii, entomopathogenic fungus, ergosterols, optimal liquid medium, optimal culture conditions |
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| 中文摘要: |
| 以古尼拟青霉生物量和麦角甾醇含量为指标，对古尼拟青霉的高产麦角甾醇培养条件及麦角甾醇提取工艺进行了优化。正交试验结果表明，高产麦角甾醇的最适液体培养基为蔗糖4%，麸皮2%，KH2PO4 0.025%和NaCl 0.05%；响应面实验结果表明，麦角甾醇最佳提取工艺为：提取溶剂浓度为80%乙醇，液料比为200:1，提取时间为65min。 |
| 英文摘要: |
| Liquid culture conditions and extraction process of ergosterols from the entomopathogenic fungus Paecilomyces gunnii were optimized by using the indices of biomass and ergosterol productions. The result of orthogonal test indicated that the optimal liquid medium contained sucrose 4%, bran 2%, KH2PO4 0.025% and NaCl 0.05%. Response surface experiment demonstrated that the optimal extract solvent concentration was 80% ethanol, the ratio of solvent to raw material was 200:1 and the extract duration was 65min. |
| 蒋岚,于士军,张莹,张琛,樊美珍,李春如. 古尼拟青霉麦角甾醇高产液体培养条件及提取工艺的优化[J]. 菌物学报, 2012, 31(3): 413-421 |
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