| 五种虫草无性型菌株产腺苷类物质的分析 |
| Analysis of adenosines in anamorphic mycelia of several species of Cordyceps |
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| 中文关键词:虫草，腺苷，虫草菌素，N6-(2-羟乙基)腺苷，检测 |
| 英文关键词:Cordyceps, cordycepin, adenosine, N6-(2 - hydroxyethyl)-adenosine, detection |
| 基金项目:贵州省社发攻关项目[黔科合SY字（2008）3047]；贵州省教育厅自然科学研究重点研究项目[黔教科（2009）0129号] |
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| 中文摘要: |
| 采用分光光度计法对虫草菌丝体中腺苷类组分的提取方法进行研究，采用薄层层析法和高效液相色谱法对虫草菌丝体中的腺嘌呤、腺苷、虫草菌素、N6-(2-羟乙基)腺苷等4种物质进行定性和定量分析。结果表明，虫草菌丝体中腺苷类组分的提取方法采用蒸馏水超声波提取30min效果较好，所测5种虫草菌株都能产腺苷和腺嘌呤，在蛹草拟青霉菌株中含有虫草菌素，在粉被玛利亚霉和蝉拟青霉菌株中含有N6-(2-羟乙基)腺苷，同时发现N6-(2-羟乙基)腺苷的累积与培养时间有一定关系。 |
| 英文摘要: |
| Spectrophotometer was used to examine different extraction method of adenosine components from anamorphic mycelia of Cordyceps species. Thin layer chromatography and high performance liquid chromatography were used to analyze qualitatively and quantitatively the adenosine components including adenine, adenosine, cordycepin and N6-(2-hydroxyethyl)-adenosine (HEA). The results showed that using distilled water ultrasonic treatment for 30min was the best extraction method. The data showed that five examined species of Cordyceps could produce adenosine and adenine. Cordyceps militaris produced cordycepin, and Cordyceps pruinosa and Cordyceps cicadae produced HEA. It was found that the incubation duration could affect the accumulation of HEA in the anamorphic mycelia. |
| 刘波,康冀川,雷帮星,何劲,文庭池,孟泽彬,潘丽丽. 五种虫草无性型菌株产腺苷类物质的分析[J]. 菌物学报, 2012, 31(3): 405-412 |
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