| 虫草素高产菌株的筛选及不同添加物对虫草素产量的影响研究 |
| Screening of high-yeild strain and medium optimization for maximum production of cordycepin by Cordyeps militaris |
| |
| 中文关键词:蛹虫草，虫草素，添加物，培养基优化 |
| 英文关键词:Cordyceps militaris, cordycepin, additives, medium optimization |
| 基金项目:湖南农业大学人才基金（No. 08YJ15）；湖南省教育厅科学研究项目（No. 09C499）；作物种质创新与资源利用重点实验室科学基金开放项目（No. 10kfxm11） |
|
| 摘要点击次数: 2831 |
| 全文下载次数: 2035 |
| 中文摘要: |
| 通过对14株蛹拟青霉菌株进行摇瓶液体发酵培养试验，筛选虫草素产量最高的蛹虫草菌株，并确定了虫草素的最佳收获期；比较8种营养添加物对虫草发酵过程中虫草素积累的影响，筛选出最佳的营养添加物，以提高液体发酵生产虫草素的产量。结果表明：蛹虫草CM001号菌株的虫草素产量最高，蛹虫草菌在第10天细胞量达到最大值20.44mg/mL，虫草素含量在第13天达到最大值73.81mg/L，70%以上的虫草素分布在发酵液中。其中分别添加腺苷、腺嘌呤、丙氨酸、L-天冬氨酸、甘氨酸5种物质，对虫草素合成的促进作用均较明显，均能有效提高蛹虫草液体发酵虫草素的产量，特别是添加1g/L的腺嘌呤能使10号菌株的虫草素产量提高7.09倍。 |
| 英文摘要: |
| In order to screen high-yield strain and determine the best harvest time of cordycepin, 14 Cordyceps militaris strains was tested by shaking flask fermentation. The effect of 8 different kinds of nutrient additives on the dynamics of cordycepin accumulation were compared. The results showed that C. militaris CM001 strain produced the highest amount of cordycepin. The biomass of C. militaris reached the maximum of 20.44mg/mL at the 10th day while the maximum cordycepin content reached 73.81mg/L at the 13th day. Seventy percent of cordycepin was in the fermentation broth. Additions of adenosine, adenine, alanine, glycine, and L-aspartic acid considerably increased the cordycepin production in the submerged culture, especially the addition of 1g/L adenine which increasing the cordycepin production by 7.09-fold than the control. |
| 王蕾,罗巍,胡瑕,刘东波,夏志兰,谢红旗. 虫草素高产菌株的筛选及不同添加物对虫草素产量的影响研究[J]. 菌物学报, 2012, 31(3): 382-388 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|