| 巴氏蘑菇多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响 |
| Effects of Agaricus blazei polysaccharides on the mRNA expression of cytokines in lead-poisoning rats |
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| 中文关键词:荧光定量，白细胞介素，肿瘤坏死因子α，γ干扰素 |
| 英文关键词:fluorescent quantitative real-time PCR, interleukin, tumor necrosis factor-α, interferon-γ |
| 基金项目:山西省自然科学基金（No. 2010011043-1）；山西农业大学博士科研启动经费项目（No. 412564）；山西省科技基础条件平台建设项目（No. 2010091017）；山西省科技攻关项目（No. 20090311047） |
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| 中文摘要: |
| 为了研究巴氏蘑菇Agaricus blazei多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响，进而探索巴氏蘑菇多糖对铅中毒大鼠的免疫调节作用。选用45日龄SD大鼠，随机分为6组，每组8只，雌雄各半。分别为正常对照组、多糖组、铅中毒模型组、铅+50mg/kg·d多糖试验组、铅+100mg/kg·d多糖试验组、铅+200mg/kg·d多糖试验组。铅中毒模型组、铅+多糖试验组分别给予含0.2%醋酸铅饮水，自由饮用。饲养60d后，采集脾脏，荧光定量RT-PCR对其细胞因子IL-1β、IL-2、IL-6、TNF-α |
| 英文摘要: |
| To investigate the immunoregulation activity of Agaricus blazei polysaccharides on lead-poisoning rats, the effects of polysaccharides on mRNA expression of cytokines in the spleen of lead-poisoning rats were studied. Forty-eight healthy 45 aged SD rats were randomly divided into six groups (female:male=1:1), and named as control group, polysaccharide group, lead-poisoning model group, lead+50mg/kg·d polysaccharide group, lead+100mg/kg·d polysaccharide group and lead+200mg/kg·d polysaccharide group respectively. The rats of lead-poisoning model group and lead+polysaccharide group gave 0.2% lead acetate water for free drink. After treatment of 60 days, the spleens were collected, and the mRNA expressions of cytokines, such as IL-1β, IL-2, IL-6, TNF-α, IFN-γ were determined with fluorescent quantitative real-time polymerase chain reaction (FQRT-PCR). The results showed that as compared with the control group, the mRNA expression levels of IL-1β, IL-2, IL-6, TNF-α, IFN-γ of polysaccharide group and lead+200mg/kg·d polysaccharide group significantly increased at 0.05 or 0.01 level, and decreased significantly at 0.01 level in lead-poisoning model group. The mRNA expression levels of the tested cytokines in lead+200mg/kg·d polysaccharide group increased at 0.01 level as compared with the lead-poisoning model group. The expression levels were incresed with the polysaccharides increased. The result suggested that Agaricus blazei polysaccharides played an improtant role in protecting the lead-induced immune system damage of animal. |
| 程红艳,常鼎然,冯翠萍,常明昌,王将,李海杰,李越. 巴氏蘑菇多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响[J]. 菌物学报, 2012, 31(2): 258-266 |
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