| COI基因作为丛赤壳科真菌DNA条形码的测试 |
| Can COI gene be used as DNA barcode for the nectriaceous fungi? |
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| 中文关键词:种内与种间差异，PCR扩增与测序成功率，邻接树 |
| 英文关键词:intra-and inter-specific variation, PCR and sequencing success rate, neighbor-joining tree |
| 基金项目:国家自然科学基金（No. 31070015）；中国科学院知识创新工程项目（No. KSCX2-EW-J-6） |
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| 中文摘要: |
| 以丛赤壳科33种76个菌株为材料，探讨COI基因作为该科DNA条形码的可能性。结果表明，该DNA片段存在较多内含子，为了获取某些种的短片段，需设计许多引物，PCR扩增与测序成功率低，难以达到便捷、快速的物种鉴定的目的。因此，COI不宜作为丛赤壳科的DNA条形码。对已获得的少数片段进行分析表明，该基因对丛赤壳科部分种具有较强的物种鉴别力。 |
| 英文摘要: |
| COI gene was tested with 76 strains of 33 species of the nectriaceous fungi to investigate its feasibility of serving as a DNA barcode. The results indicated that introns are present in some species, which made it difficult for PCR amplification and sequencing. COI gene is not a good DNA barcode for this group. Analysis of the obtained COI sequences showed that this gene may have the capacity to distinguish species of the nectriaceous fungi. |
| 赵鹏,罗晶,庄文颖. COI基因作为丛赤壳科真菌DNA条形码的测试[J]. 菌物学报, 2012, 31(2): 243-250 |
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