| 新型隐球酵母氯离子通道在阳离子内稳态中的趋异作用 |
| Divergent roles of CLC1 in cation homeostasis in Cryptococcus neoformans |
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| 中文关键词:漆酶，阳离子，功能差异 |
| 英文关键词:laccase, cation, divergent function |
| 基金项目:国家自然科学基金（No. 30770043）；国家“973项目”（No. 2007CB707801） |
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| 中文摘要: |
| 新型隐球酵母Cryptococcus neoformans有两个变种（varieties），即grubii和neoformans。目前研究最多的两个菌株H99（血清型A）和JEC21（血清型D）分别代表这两个变种，两者的毒性差别显著，为研究新型隐球酵母菌株间毒性的进化提供了良好模型。我们通过比较JEC21的clc1-突变体Tx1与早先鉴定的H99 clc1-菌株Mlac3发现，JEC21 CLC1同样决定铜离子的吸收。Tx1中丧失的漆酶活力可以通过外源Cu2+的加入得以恢复，而漆酶基因LAC1的转录与野生 |
| 英文摘要: |
| Cryptococcus neoformans has two varieties, var. grubii and var. neoformans. Two well-studied strains H99 (serotype A) and JEC21 (serotype D) are representatives of var. grubii and var. neoformans respectively. The virulence of the two strains is different which provides an ideal model for researching the evolution of virulence. By comparing the JEC21-derived clc1- mutant strain Tx1 and previously reported H99-derived clc1- mutant strain Mlac3, it was found that CLC1 also participated in copper uptake in JEC21. The demolished laccase activity in Tx1 strain was restored with exogenous copper, while the transcription of the laccase gene LAC1 was at a comparable level to JEC21. Compared with the wild-type strain, the transcription of the copper transporter gene CTR4 was highly upregulated in Tx1 which was not repressed by exogenous copper obviously. These data revealed a lack of intracellular copper in Tx1 cells. In contrast, Tx1 was shown to be hypersensitive to 1mol/L Na+/K+, while Mlac3 was not. Tx1 was viable under pH8.0 condition, whereas Mlac3 was sensitive to pH8.0. The results demonstrated that CLC1 shared a common role in Cu2+ homeostasis, but divergent roles in regulating Na+, K+ and H+ between H99 and JEC21. This work may provide support to the virulence difference between H99 and JEC21. |
| 李东,张笑娇,杨娇,潘皎,朱旭东. 新型隐球酵母氯离子通道在阳离子内稳态中的趋异作用[J]. 菌物学报, 2012, 31(2): 235-242 |
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