| 分离自青海湖可培养耐盐真菌的多样性研究 |
| Diversity of halotolerant fungi isolated from Qinghai Lake based on culture-dependent investigation |
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| 中文关键词:青藏高原，极端微生物，菌种鉴定 |
| 英文关键词:Qinghai-Tibet Plateau, extremophilic microorganisms, strain identification |
| 基金项目:国家自然科学基金（No. 30960014）；大学生科技创新项目（No. 101074301）；青海省科技厅项目（No. 2011-Z-14） |
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| 中文摘要: |
| 青海湖位于青藏高原东北部，属于典型的高原盐湖沉积环境。作者以常规的分离培养方法对青海湖中的耐盐真菌多样性进行了研究。从黑马河、耳海和尕海共分离得到617株耐盐真菌，结合形态学和分子生物学的方法将菌株鉴定到18个属和45个种。子囊菌的菌株数比接合菌和担子菌多，枝孢菌和青霉菌为优势耐盐真菌。在这45种耐盐真菌中，轻度、中度和极端嗜盐真菌分别有19、25和1种。分离自黑马河地区的耐盐真菌，其物种丰富度指数Ma（3.12）、多样性指数H′（1.11）和均匀度指数J（0.267）均比耳海和尕海的高。从1.5%浓度下 |
| 英文摘要: |
| Qinghai Lake, a typical depositional salt lake, is located in the northeast of Qinghai-Tibet Plateau. In this paper, halotolerant fungi of Qinghai Lake were investigated by traditional culture-dependent methods. Totally, 617 fungal cultures were isolated from different regions which named as Heima Lake, Erhai and Gahai in Qinghai Lake. They were assigned to 18 genera and 45 species based on morphological and molecular characteristics. The number of isolates of Ascomycetes was larger than that of Basidiomycetes and Zygomycetes, and the most abundant genera were Cladosporium and Penicillium. 19 species and 25 species were the slight halophiles and moderate halophiles, respectively, while only one species was extreme halophiles. The all values of Ma (3.12), H′ (1.11) and J (0.267) of halophiles isolated from Heima Lake were larger than those of Erhai and Gahai in all salinity; the all values of Ma (3.04), H′ (1.13) and J (0.293) of halophiles isolated from 1.5% salinity were higher than those from 10% and 30% salinity, and the H′ (1.13) and J (0.276) of halophiles isolated from water samples were higher than those from the mud and soil, while the Ma (3.10) of halophiles isolated from water samples was lower than that from mud. This is a systematic report on the diversity of halotolerant fungi from Qinghai Lake by culture-dependent methods. |
| 谢占玲,王欢,赵朋,余静,吕超,申贤慧,龚宝林,刘英. 分离自青海湖可培养耐盐真菌的多样性研究[J]. 菌物学报, 2012, 31(2): 187-195 |
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