| 草菇聚酮合酶编码基因vv-alb的克隆及其表达的初步分析 |
| Cloning and expression analysis of a polyketide synthase gene vv-alb from the edible straw mushroom, Volvariella volvacea |
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| 中文关键词:草菇，聚酮合酶，荧光实时定量RT-PCR |
| 英文关键词:Volvariella volvacea, polyketide synthase, real-time quantitative PCR |
| 基金项目:上海市农业科学院青年基金[农青年科技2009（09）] |
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| 中文摘要: |
| 聚酮化合物（polyketides）是一类庞大的次级代谢家族，聚酮合酶（polyketide synthase，PKS）是介导聚酮化合物生物合成的关键酶。通过巢氏简并PCR与染色体步行的方法，获得了草菇中的编码PKS的基因vv-alb的全长序列，并通过荧光实时定量RT-PCR方法对vv-alb基因在草菇不同生长阶段与不同部位的表达情况进行了初步分析，为进一步研究PKS在草菇和其他食用真菌生物代谢过程中的作用奠定了一定的基础。 |
| 英文摘要: |
| Polyketides are secondary metabolites that exhibit a broad range of biological activities. The key enzyme involved in biosynthesis is polyketide synthase (PKS). We report the cloning of a putative PKS gene (vv-alb) by nested PCR using degenerate primers and chromosome walking from the edible straw mushroom, Volvariella volvacea. The gene vv-alb is 6,704bp in size and contains a 6,279bp coding region (encoding 2,093 amino acid residues) and eight introns. The encoded product of the vv-alb gene contains four functional domains: ketosynthase (KS), acyl transferase (AT), thioesterase (TE) and acyl carrier protein (ACP), the typical characteristics of PKS. Real-time quantitative PCR analysis revealed that vv-alb expression was 5-10-fold higher in the stipe region as compared with the vegetative mycelium, primordia and pileus. |
| 林楠,茅文俊,汪虹,冯爱萍,鲍大鹏. 草菇聚酮合酶编码基因vv-alb的克隆及其表达的初步分析[J]. 菌物学报, 2012, 31(1): 142-149 |
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